Molecular Environmental Microbiology Laboratory

deLorenzoVRed

 

Victor de Lorenzo

Group Leader

Contact

 

Research summary

Our laboratory is committed to understanding how bacteria that inhabit natural niches sense and process multiple environmental signals into distinct responses –both at the level of single cells and as a community. Unlike laboratory settings, in which growth conditions can be controlled and changed one at a time, bacteria in the environment must perpetually make decisions between activating metabolic genes for available, frequently mixed C-sources and those for escaping or adapting to physicochemical stress.

 

Publications

Martínez-García E, Aparicio T, Goñi-Moreno A, Fraile S, de Lorenzo V. SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities. Nucl Acids Res 2014; 43: D1183-D1189

Nikel PI, Martínez-García E, de Lorenzo V. Biotechnological domestication of pseudomonads using synthetic biology. Nat Microbiol Rev 2014; 12: 368-379

Calles B, de Lorenzo V. Expanding the Boolean logic of the prokaryotic transcription factor XylR by functionalization of permissive sites with a protease-target sequence. ACS Synthetic Biol 2013; 2: 594–603

Pérez-Pantoja D, Nikel PI, Chavarría M, de Lorenzo, V. Endogenous stress caused by faulty oxidation reactions fosters evolution of 2,4-dinitrotoluene-degrading bacteria. PLoS Genetics 2013; 9: e1003764

Funding

logo ministerio eco com FEDER

 

 

 

Convocatoria Retos-Colaboración 2014, RTC-2014-1777-3: Desarrollo de herramientas metagenómicas para producción de biocombustibles (CAMBIOS).

 

FigVictordLorenzo

 

The longstanding aim of our team is to produce biological agents for biosensing, remediation and (wherever possible) transformation of urban and industrial chemical waste that is otherwise dumped into the environment. To this end, we explore and capitalize on the interface between environmental microbiology and synthetic biology. Our workhorse is the soil bacterium Pseudomonas putida, which combines the ease of genetic programming typical of Escherichia coli with the safety, robustness and metabolic abilities required of whole-cell catalysts for applications in harsh biotechnological settings. Specific activities include [i] development of P. putida as a reliable chassis for implantation of genetic and metabolic circuits. This involves editing the extant genome of this microorganism to enhance desirable properties and reduce drawbacks. We also use surface-display systems to design complex catalytic properties completely separate from the cell metabolism, as well as artificial communities by expression of ectopic adhesins. [ii] Use of camel antibodies as tools for metabolic and regulatory engineering of P. putida. Various expression systems allow the targeting of camelid VHH fragments to the intracellular compartment, the periplasm or the external medium of the cells. This allows selective perturbations of chosen metabolic routes to enhance desired metabolic and regulatory qualities of cells. [iii] Genetic tools for deep refactoring of P. putida metabolic properties. The new assets we are developing include a large collection of standardised plasmid and transposon vectors, as well as dedicated reporter systems for parameterisation of the gene expression flow and for switching entire metabolic regimes. [iv] The TOL system borne by plasmid pWW0 as a reference for metabolic circuit implantation. The two operons for toluene and m xylene biodegradation encoded in pWW0 offer a natural case of expansion of the metabolic repertoire of environmental bacteria through acquisition of new genes. [v] Deep metabolic engineering of P. putida. Current efforts attempt to develop strains programmed to adopt an entirely anoxic metabolism and/or a genuine glycolytic mode of utilizing glucose. This involves the re-engineering of dozens of genes and metabolic modules.

 

 

grupoDeLorenzo 

Name
Position
Contact
Víctor de Lorenzo Principal investigator
Yamal Al-Ramahí Postdoctoral scientist
Ilaria Benedetti Postdoctoral scientist
Belén Calles Postdoctoral scientist
Angel Goñi Postdoctoral scientist
Esteban Martínez Postdoctoral scientist
Pablo I. Nikel Postdoctoral scientist
Mª de los Angeles Hueso Predoctoral scientist
Alberto Sánchez-Pascuala Predoctoral scientist
Sofia Fraile Technicians
Tomás Aparicio Technicians
Inés Merino Administrative assistant

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