Molecular Environmental Microbiology Laboratory

VLorenzoVBaja

 

Victor de Lorenzo

Group Leader

Contact

 

Research summary

Our laboratory is committed to understanding how bacteria that inhabit natural niches sense and process multiple environmental signals into distinct responses –both at the level of single cells and as a community. Unlike laboratory settings, in which growth conditions can be controlled and changed one at a time, bacteria in the environment must perpetually make decisions between activating metabolic genes for available, frequently mixed C-sources and those for escaping or adapting to physicochemical stress.

 

Publications

Aparicio T, Silbert J, Cepeda S Lorenzo V. Propagation of recombinant genes through complex microbiomes with synthetic mini-RP4 plasmid vectors. BioDesign Res 2022, 2022 ID: 9850305

Velázquez E, Álvarez B, Fernández LA, de Lorenzo V. Hypermutation of specific genomic loci of Pseudomonas putida for continuous evolution of target genes. Microb Biotechnol 2022 15(9):2309-2323

Kim J, Silva-Rocha R, de Lorenzo V. Picking the right metaphors for addressing microbial systems: economic theory helps understanding biological complexity. Int Microbiol. 2021 Nov;24(4):507-519.

Espeso DR, Dvořák P, Aparicio T, de Lorenzo V. An automated DIY framework for experimental evolution of Pseudomonas putida. Microb Biotechnol. 2021 Nov;14(6):2679-2685.

Velázquez E, Al-Ramahi Y, Tellechea-Luzardo J, Krasnogor N, de Lorenzo V. Targetron-Assisted Delivery of Exogenous DNA Sequences into Pseudomonas putida through CRISPR-Aided Counterselection ACS Synth Biol. 2021 Oct 15;10(10):2552-2565.

Al-Ramahi Y, Nyerges A, Margolles Y, Cerdán L, Ferenc G, Pál C, Fernández LÁ, de Lorenzo V. ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces. Commun Biol. 2021 Oct 7;4(1):1169.

Tas H, Grozinger L, Goñi-Moreno A, de Lorenzo V. Automated design and implementation of a NOR gate in Pseudomonas putida Synth Biol (Oxf). 2021 Sep 1;6(1):ysab024.


More publications

 

Funding

Ministerio de Ciencia innovacion FEDER

 

 

 

RTI2018-095584-B-C42. Programación genética de Pseudomonas putida para una eficacia catalítica sostenida a través de la escala de disponibilidad de agua.

 

 

The longstanding mission our team is the production of biological agents for biosensing, large-scale remediation and valorisation of chemical waste that is otherwise dumped into the Environment by urban and industrial activities. The workhorse to this end is the soil bacterium Pseudomonas putida, which combines the ease of genetic programming that is typical of Escherichia coli with the safety, robustness and metabolic capabilities required in whole-cell catalysts for applications in harsh biotechnological settings.

de Lorenzo figure1

Specific activities include:

[i] Development of P. putida as a reliable chassis for implantation of genetic and metabolic circuits. This involves a profound editing of the extant genome of this microorganism for enhancing desirable properties and eliminating drawbacks. Also, the exploitation of surface-display systems for designing complex catalytic properties altogether separated from the cell metabolism and even the design of artificial communities by means of ectopic adhesins.

[ii] Genetic tools for deep refactoring of metabolic properties of P. putida. The list of new assets that we are developing includes a large collection of standardized plasmid and transposon vectors as well as dedicated reporter systems for parameterization of the gene expression flow and for switching entire metabolic regimes.

[iii] The TOL system borne by plasmid pWW0 as a natural example of well-nested metabolic circuit implantation. The two operons for toluene and m-xylene biodegradation encoded in pWW0 offer a case of expansion of the metabolic repertoire of environmental bacteria through acquisition of new genes.

[iv] Deep metabolic engineering of P. putida. Currents efforts attempt to develop strains that can be entirely programmed to deliver catalytic phenotypes of choice upon exposure and computation of both external and internal cues. This endeavour combines direct rational engineering with fine-tuning of gene expression by means of site-specific diversification of genomic sequences of choice through adaptation to P. putida of high-efficacy genome engineering technology


 

 

deLorenzo group 2022 

Name
Position
Contact
Víctor de Lorenzo Principal investigator
Belén Calles Senior Scientist
Esteban Martínez Postdoctoral scientist
David Rodríguez Espeso Postdoctoral scientist
Tomás Aparicio Postdoctoral scientist
Elena Velázquez Postdoctoral scientist
Elena Algar Postdoctoral scientist
Guillermo Gómez Postdoctoral scientist
Francisco Moreno Predoctoral scientist
Sofia Fraile Technicians
Luis Almonacid Technicians
Inés Merino Administrative assistant

NOTE! This site uses cookies and similar technologies.

If you continue browsing or do not change browser settings, we consider your acepptance for using. Learn more

I understand

COOKIES POLICY

A cookie is a text file that is stored on your computer or mobile device via a web server and only that server will be able to retrieve or read the contents of the cookie and allow the Web site remember browser preferences and navigate efficiently. Cookies make the interaction between the user and the website faster and easier.

General information

This Website uses cookies. Cookies are small text files generated by the web pages you visit, which contain the session data that can be useful later in the website. In this way this Web remembers information about your visit, which can facilitate your next visit and make the website more useful.

How do cookies?

Cookies can only store text, usually always anonymous and encrypted. No personal information is ever stored in a cookie, or can be associated with identified or identifiable person.

The data allow this website to keep your information between the pages, and also to discuss how to interact with the website. Cookies are safe because they can only store information that is put there by the browser, which is information the user entered in the browser or included in the page request. You can not run the code and can not be used to access your computer. If a website encrypts cookie data, only the website can read the information.

What types of cookies used?

The cookies used by this website can be distinguished by the following criteria:

1. Types of cookies as the entity that manages:

Depending on who the entity operating the computer or domain where cookies are sent and treat the data obtained, we can distinguish:

- Own cookies: are those that are sent to the user's terminal equipment from a computer or domain managed by the editor itself and from which provides the service requested by the user.

- Third party cookies: these are those that are sent to the user's terminal equipment from a machine or domain that is not managed by the publisher, but by another entity data is obtained through cookies.

In the event that the cookies are installed from a computer or domain managed by the editor itself but the information collected by these is managed by a third party can not be considered as party cookies.

2. Types of cookies as the length of time that remain active:

Depending on the length of time that remain active in the terminal equipment can be distinguished:

- Session cookies: cookies are a type designed to collect and store data while the user accesses a web page. Are usually used to store information that only worth preserving for the service requested by the user at any one time (eg a list of products purchased).

- Persistent cookies: cookies are a type of data which are stored in the terminal and can be accessed and treated for a period defined by the head of the cookie, and can range from a few minutes to several years.

3. Cookies types according to their purpose:

Depending on the purpose for which the data are processed through cookies, we can distinguish between:

- Technical cookies: these are those that allow the user to navigate through a web page or application platform and the use of different options or services it exist as, for example, control traffic and data communication, identify the session, access to restricted access parts, remember the elements of an order, make the buying process an order, make an application for registration or participation in an event, use security features while browsing store content for dissemination videos or sound or share content via social networks.

- Customization cookies: these are those that allow the user to access the service with some general characteristics based on a predefined set of criteria in the user terminal would eg language, the type of browser through which you access the service, the locale from which you access the service, etc.

- Analysis cookies: they are those that allow the responsible for them, monitoring and analyzing the behavior of users of the web sites that are linked. The information gathered through such cookies are used in measuring the activity of web sites, application or platform and for the profiling of user navigation of such sites, applications and platforms, in order to make improvements function data analysis how users use the service.

Management tool cookies

This Website uses Google Analytics.

Google Analytics is a free tool from Google that primarily allows website owners know how users interact with your website. Also, enable cookies in the domain of the site in which you are and uses a set of cookies called "__utma" and "__utmz" to collect information anonymously and reporting of website trends without identifying individual users..

For statistics of use of this website use cookies in order to know the level of recurrence of our visitors and more interesting content. This way we can concentrate our efforts on improving the most visited areas and make the user more easily find what they are looking for. On this site you can use the information from your visit for statistical evaluations and calculations anonymous data and to ensure the continuity of service or to make improvements to their websites. For more details, see the link below privacy policy [http://www.google.com/intl/en/policies/privacy/]

How to manage cookies on your computer: disabling and deleting cookies

All Internet browsers allow you to limit the behavior of a cookie or disable cookies within settings or browser settings. The steps for doing so are different for each browser, you can find instructions in the help menu of your browser.

If you decline the use of cookies, since it is possible thanks to the preferences menu of your browser or settings, reject, this website will continue to function properly without the use of the same.

Can you allow, block or delete cookies installed on your computer by setting your browser options installed on your computer:

- For more information about Internet Explorer click here.
- For more information on Chrome click here.
- For more information about Safari click here.
- For more information about Firefox click here.

Through your browser, you can also view the cookies that are on your computer, and delete them as you see fit. Cookies are text files, you can open and read the contents. The data within them is almost always encrypted with a numeric key corresponding to an Internet session so often has no meaning beyond the website who wrote it.

Informed consent

The use of this website on the other hand, implies that you paid your specific consent to the use of cookies, on the terms and conditions provided in this Cookies Policy, without prejudice to the measures of deactivation and removal of cookies that you can take, and mentioned in the previous section.