Plant-pathogen interaction in viral infections

Garcia

 ERojo

Juan Antonio García

Group Leader

Contact

 

Carmen Simón

Group Leader

Contact

 

 

Research summary

Plant viruses depend largely on host factors to replicate in the cell and to propagate throughout the plant and between individual plants. Plants in turn have developed antiviral defence mechanisms that must be counteracted by viral factors. These factors appear to be preferred targets for alternative plant defences. In our laboratory, we try to understand this complex interplay, mainly in the infection of the potyvirus Plum pox virus (PPV), the causal agent of sharka, a damaging disease of Prunus trees. We are especially interested in defence responses related to RNA silencing and its viral suppressors.

 

Publications

Pasin F, Bedoya LC, Bernabé-Orts JM, Gallo A, Simón-Mateo C, Orzaez D, García JA. Multiple T-DNA delivery to plants using novel mini binary vectors with compatible replication originsACS Synth Biol 2017 Oct 20;6(10):1962-1968

Gallo A, Valli A, Calvo M, García JA. A functional link between RNA replication and virion assembly in the potyvirus Plum pox virus. J. Virol. 2018; 92, e02179-17.

Ochoa J, Valli A, Martín-Trillo M, Simón-Mateo C, García JA, Rodamilans B. Sterol isomerase HYDRA1 interacts with RNA silencing suppressor P1b and restricts potyviral infection. Plant Cell Environ 2019; 42: 3015-3026

Hervás M, Navajas R, Chagoyen M, Garcia JA, Martinez-Turiño, S. Phosphorylation-related cross-talk between distant regions of the core region of the coat protein contributes to virion assembly of Plum pox virus. . Mol Plant Microbe Interact 2020; 33: 653-667

González de Prádena A, Sánchez Jiménez A, San León D, Simmonds P, García JA, Valli AA. Plant virus genome is shaped by specific dinucleotide restrictions that influence viral infectionMBio 2020; 11: e02818-02819

 

Fig.2RedA complex plant-virus interactive network modulates infectivity and symptom severity. Most plant viruses cannot infect all their potential hosts, and when they do, serious illness is not the norm, as revealed by recent metagenomic studies. Our laboratory studies this interaction network, which facilitates virus replication and propagation, but also induces plant defense responses and disease symptoms. Plum pox virus, our main subject of study, belongs to the family Potyviridae, the largest group of plant RNA viruses and causes sharka, a serious disease of stone fruit trees.

We are especially interested in defense responses related to RNA silencing and its viral suppressors. The typical silencing suppressor of potyvirids is HCPro, but the existence of additional silencing suppressors in different potyvirids, prompted us to suggest that escaping RNA silencing-mediated antiviral defenses is a powerful driving force of virus evolution. Potyvirid genomic RNAs are expressed through the synthesis of large polyproteins, processed by viral-encoded endopeptidases.

We are studying how host-specific modulation of this processing can contribute to potyviral pathogenicity and host range definition. Encapsidation of potyvirid genome is an active process. We have demonstrated a functional link between potyvirus RNA replication and virion assembly, and we are studying how posttranslational modifications of the capsid protein can contribute to sort the potyviral RNA into translation, replication or encapsidation. An important goal of our laboratory is applying our basic research results to control viral diseases through novel strategies. For instance, we are attenuating plant viruses by recoding their genomes in order to use them as cross-protection agents. We are also interested in developing other valuable biotools, such as a novel T-DNA delivery system, which can be used to efficiently inoculate plants with infectious viral cDNA clones, among other multiple applications.

  Fig.1Red

 

Garcia simon grupo 2018 Laboratory Members

Name
Position
Contact
Juan Antonio García Principal investigator
Carmen Simón Principal investigator
Adrian Valli Ramón y Cajal researcher
Bernardo Rodamilans Postdoctoral scientist
Sandra Martínez Turiño Postdoctoral scientist
Alfonso González de Prádena Predoctoral scientist
Rafael García Predoctoral scientist
Beatriz García Technician
Irene Gonzalo Technician

 

Adrian A. Valli

Ramón y Cajal grant call: 2018

Project´s name: Plant-Virus Coevolution

Summary: With a world population estimated in 9.6 billion people by mid-century, there is a pressing need to improve food security, with crop protection measures being essential components of any strategy that aims to reduce yield losses. RNA viruses are among the most abundant and economically relevant pathogens infecting plants; indeed, they cause more than 50% of viral crop damage worldwide. Gaining insight about this group of viruses is then critical to reveal and understand not only new features of them, but also to discover novel plant protein networks acting as defensive barriers. Thanks to these basic studies, more and better antiviral strategies are being developed and implemented for the protection of important crops. Intriguingly, despite the importance of plant RNA viruses for food security, it is surprising to find that very little is known about their RNA-dependent RNA polymerases (RdRPs), putative RdRP protein partners and the precise role/s of these partners during infection.

As a relevant socio-economical case we currently study the partnership between RdRP and the pyrophosphatase HAM1 deriving from Ugandan cassava brown streak virus, one the agents causing the “Ebola of plants” in cassava (the fourth most important crop on earth based on calorie intake). To do that we follow a multidisciplinary approach that includes (i) synthetic biology to build chimerical infectious clones, (ii) genomics studies of virus mutants to define quasispecies variability, (iii) structural studies by cryo-electron microscopy to define protein 3D structures, (iv) metabolomics studies by HPLC-MS/MS to understand the viral disease, and (v) viral ecology to decipher the interaction between cassava (and other plants from the Euphorbiaceae family) and viruses in nature. These approaches will greatly help us to fill gaps in our understanding of RdRPs in general, as well as RdRP-HAM1 partnership in viruses infecting plants from the Euphorbiaceae family.

Figura3

Figure Legend: Infection of plants with laboratory-designed cDNA clones of UCBSV. (A) The RNA genome of UCBSV was retrotranscribed to cDNA and this sequence was then inserted in between a strong promoter (p35S from Cauliflower mosaic virus) and a terminator (tNOS from Agrobacterium nopaline synthase) to generate the infectious cDNA plasmid termed pLX-UCBSV (Pasin et al., 2017, ACS Synthetic Biology). This plasmid was further modified to tag the virus with GFP, thus generating pLX-UCBSV-GFP. (B) Leaves of cassava plants (Manihot esculenta) at 2 months post-inoculation observed under white light (bar = 4 cm). Symptoms of infection are clearly detected in upper leaves of plants inoculated with pLX-UCBSV. (C) One leaf of a Nicotiana benthamiana plant, infected with UCBSV-GFP, observed under white light and UV light (to detect GFP-derived fluorescence) with a stereomicroscope. GFP is distributed across primary and secondary veins as the virus spread through the leaf (bar = 0.5 cm).

Laboratory Members

Name
Position
Contact
Adrian Valli Ramón y Cajal researcher
Alfonso González de Prádena Predoctoral scientist, co-supervised with Juan Antonio García
Rafael García Predoctoral scientist
Irene Gonzalo Technician
Julio César Aragón Lago Graduate Student JAE-INTRO, from Sept 2020 to Feb 2021

Funding

Uncovering the mechanism and potential uses of viral RNA polymerase slippage. Spanish MICINN (BIO2015-73900-JIN), from 2017 to 2019 (3 years).

Ramón y Cajal Program (RYC2018-025523-I). Spanish MICINN, from 2020 to 2024 (5 years)..

The paradigm of Euphorbiaceous-Virus interaction: a multidisciplinary approach to develop novel antiviral strategies in plants. Spanish MICINN (PID2019-110979RB-I00), from 2020 to 2022 (3 years).

The Euphorbiaceous-Virus Coevolution. Spanish Research Council (CSIC), from 2020 to 2022 (3 years).

NOTE! This site uses cookies and similar technologies.

If you continue browsing or do not change browser settings, we consider your acepptance for using. Learn more

I understand

COOKIES POLICY

A cookie is a text file that is stored on your computer or mobile device via a web server and only that server will be able to retrieve or read the contents of the cookie and allow the Web site remember browser preferences and navigate efficiently. Cookies make the interaction between the user and the website faster and easier.

General information

This Website uses cookies. Cookies are small text files generated by the web pages you visit, which contain the session data that can be useful later in the website. In this way this Web remembers information about your visit, which can facilitate your next visit and make the website more useful.

How do cookies?

Cookies can only store text, usually always anonymous and encrypted. No personal information is ever stored in a cookie, or can be associated with identified or identifiable person.

The data allow this website to keep your information between the pages, and also to discuss how to interact with the website. Cookies are safe because they can only store information that is put there by the browser, which is information the user entered in the browser or included in the page request. You can not run the code and can not be used to access your computer. If a website encrypts cookie data, only the website can read the information.

What types of cookies used?

The cookies used by this website can be distinguished by the following criteria:

1. Types of cookies as the entity that manages:

Depending on who the entity operating the computer or domain where cookies are sent and treat the data obtained, we can distinguish:

- Own cookies: are those that are sent to the user's terminal equipment from a computer or domain managed by the editor itself and from which provides the service requested by the user.

- Third party cookies: these are those that are sent to the user's terminal equipment from a machine or domain that is not managed by the publisher, but by another entity data is obtained through cookies.

In the event that the cookies are installed from a computer or domain managed by the editor itself but the information collected by these is managed by a third party can not be considered as party cookies.

2. Types of cookies as the length of time that remain active:

Depending on the length of time that remain active in the terminal equipment can be distinguished:

- Session cookies: cookies are a type designed to collect and store data while the user accesses a web page. Are usually used to store information that only worth preserving for the service requested by the user at any one time (eg a list of products purchased).

- Persistent cookies: cookies are a type of data which are stored in the terminal and can be accessed and treated for a period defined by the head of the cookie, and can range from a few minutes to several years.

3. Cookies types according to their purpose:

Depending on the purpose for which the data are processed through cookies, we can distinguish between:

- Technical cookies: these are those that allow the user to navigate through a web page or application platform and the use of different options or services it exist as, for example, control traffic and data communication, identify the session, access to restricted access parts, remember the elements of an order, make the buying process an order, make an application for registration or participation in an event, use security features while browsing store content for dissemination videos or sound or share content via social networks.

- Customization cookies: these are those that allow the user to access the service with some general characteristics based on a predefined set of criteria in the user terminal would eg language, the type of browser through which you access the service, the locale from which you access the service, etc.

- Analysis cookies: they are those that allow the responsible for them, monitoring and analyzing the behavior of users of the web sites that are linked. The information gathered through such cookies are used in measuring the activity of web sites, application or platform and for the profiling of user navigation of such sites, applications and platforms, in order to make improvements function data analysis how users use the service.

Management tool cookies

This Website uses Google Analytics.

Google Analytics is a free tool from Google that primarily allows website owners know how users interact with your website. Also, enable cookies in the domain of the site in which you are and uses a set of cookies called "__utma" and "__utmz" to collect information anonymously and reporting of website trends without identifying individual users..

For statistics of use of this website use cookies in order to know the level of recurrence of our visitors and more interesting content. This way we can concentrate our efforts on improving the most visited areas and make the user more easily find what they are looking for. On this site you can use the information from your visit for statistical evaluations and calculations anonymous data and to ensure the continuity of service or to make improvements to their websites. For more details, see the link below privacy policy [http://www.google.com/intl/en/policies/privacy/]

How to manage cookies on your computer: disabling and deleting cookies

All Internet browsers allow you to limit the behavior of a cookie or disable cookies within settings or browser settings. The steps for doing so are different for each browser, you can find instructions in the help menu of your browser.

If you decline the use of cookies, since it is possible thanks to the preferences menu of your browser or settings, reject, this website will continue to function properly without the use of the same.

Can you allow, block or delete cookies installed on your computer by setting your browser options installed on your computer:

- For more information about Internet Explorer click here.
- For more information on Chrome click here.
- For more information about Safari click here.
- For more information about Firefox click here.

Through your browser, you can also view the cookies that are on your computer, and delete them as you see fit. Cookies are text files, you can open and read the contents. The data within them is almost always encrypted with a numeric key corresponding to an Internet session so often has no meaning beyond the website who wrote it.

Informed consent

The use of this website on the other hand, implies that you paid your specific consent to the use of cookies, on the terms and conditions provided in this Cookies Policy, without prejudice to the measures of deactivation and removal of cookies that you can take, and mentioned in the previous section.