Genetic stability

JCAlonso

 

Juan Carlos Alonso

Group Leader

Contact

 

Research summary

Nuestros objetivos son caracterizar la implicación de las funciones de reparación del ADN y la segregación cromosomal en la estabilidad del material genético en firmicutes. Para ello se utilizan dos modelos experimentales: Bacillus subtilis se usa para estudiar el papel de las proteínas de recombinación en la mecánica de la reparación de ADN y el plásmido pSM19035 para estudiar a la estabilidad segregacional.

 

Publications

Carrasco B, Escobedo S, Alonso JC, Suárez JE Modulation of Lactobacillus casei bacteriophage A2 lytic/lysogenic cycles by binding of Gp25 to the early lytic mRNA. Mol Microbiol 2016; 99: 328-337

Carrasco B, Serrano E, Sánchez H, Wyman C, Alonso JC. Chromosomal transformation in Bacillus subtilis is a non-polar recombination reaction. Nucleic Acids Res 2016 44: 2754-2768

Volante A, Carrasco B, Tabone M, Alonso JC. The interaction of omega with the RNA polymerase delta’ subunit functions as an activation to repression switch. Nucleic Acids Res 2015; 43: 9249-9261

Carrasco B, Yadav T, Serrano E, Alonso JC. Bacillus subtilis RecO and SsbA are crucial for RecA-mediated recombinational DNA repair. Nucleic Acids Res 2015; 43: 5984-5997

Volante A, Alonso JC. Molecular anatomy of ParA-ParA and ParA-ParB interactions during plasmid partitioning. J Biol Chem 2015; 290: 18782-95

 

Fig1AlonsoOur research focuses on the study of the molecular mechanisms that bacteria of the phylum Firmicutes use to secure the stability and horizontal transfer of genetic information, and to promote accurate segregation. Bacillus subtilis cells are chosen to study repair-by-recombination and plasmid segregation stability during vegetative growth as well as genetic recombination during natural competence. Using this model, we showed that the DNA damage response recruits different complex molecular machineries depending of the type of DNA damage, double-strand breaks (DSB) or single-strand gaps (SSG).

i) RecN, which is among the first responders to DSB, in concert with PNPase, promotes dynamic recruitment of DNA ends onto a repair centre. The AddAB complex or RecJ, in concert with the RecQ or RecS helicase and SsbA, creates a 3’-single-stranded (ss)DNA tailed duplex at the DNA breaks, and RecN recruits RecA mediators (SsbA, RecOR) and modulators (RecF, RecX, RecU, RecD) to initiate recombinational DNA repair (Fig. 1)

ii) distinct accessory proteins (DprA and SsbA and RecO(R) and SsbA) regulate recruitment and activation of RecA in the ATP-bound form to repair DSB and SSG (Fig. 1)

iii) CdaA and c-di-AMP are also involved in DSB repair, although their role is poorly understood

iv) in concert with RadA/Sms and c-di-AMP, DisA recognises recombination intermediates and modulates the repair of reversed forks by a poorly understood mechanism. Studying segregation in this bacterium, we showed that a) low copy number plasmids require homodimeric ParA-like (d2) and ParB-like (w2) parS regions for stable inheritance at cell division, b) the d2-ATP protein associates dynamically to the nucleoid, c) d2 bound to the nucleoid captures and tethers plasmid copies towards the nucleoid, d) at stoichiometric d2 and w2 concentrations, the latter facilitates ATP hydrolysis, creating a gradient of nucleoid-bound self-organizing d2 clouds, e) ATP hydrolysis leads to d2 disassembly from DNA, and the dynamic assembly/disassembly moves the plasmid molecule towards the cells poles to guarantee faithful segregation;, and f) if segregation fails, the toxin-antitoxin system halts proliferation of plasmid-free cells, with subsequent overgrowth of plasmid-bearing cells.

 

Fig2Alonso

 

 grupoAlonsoRed

 

Name
Position
Contact
Juan C. Alonso Principal investigator
Begoña Carrasco Postdoctoral scientist
Ester Serrano Predoctoral scientists
Mariangela Tabone Predoctoral scientists
Andrea Volante Predoctoral scientists
Chiara Marchisone Technician

NOTE! This site uses cookies and similar technologies.

If you continue browsing or do not change browser settings, we consider your acepptance for using. Learn more

I understand

COOKIES POLICY

A cookie is a text file that is stored on your computer or mobile device via a web server and only that server will be able to retrieve or read the contents of the cookie and allow the Web site remember browser preferences and navigate efficiently. Cookies make the interaction between the user and the website faster and easier.

General information

This Website uses cookies. Cookies are small text files generated by the web pages you visit, which contain the session data that can be useful later in the website. In this way this Web remembers information about your visit, which can facilitate your next visit and make the website more useful.

How do cookies?

Cookies can only store text, usually always anonymous and encrypted. No personal information is ever stored in a cookie, or can be associated with identified or identifiable person.

The data allow this website to keep your information between the pages, and also to discuss how to interact with the website. Cookies are safe because they can only store information that is put there by the browser, which is information the user entered in the browser or included in the page request. You can not run the code and can not be used to access your computer. If a website encrypts cookie data, only the website can read the information.

What types of cookies used?

The cookies used by this website can be distinguished by the following criteria:

1. Types of cookies as the entity that manages:

Depending on who the entity operating the computer or domain where cookies are sent and treat the data obtained, we can distinguish:

- Own cookies: are those that are sent to the user's terminal equipment from a computer or domain managed by the editor itself and from which provides the service requested by the user.

- Third party cookies: these are those that are sent to the user's terminal equipment from a machine or domain that is not managed by the publisher, but by another entity data is obtained through cookies.

In the event that the cookies are installed from a computer or domain managed by the editor itself but the information collected by these is managed by a third party can not be considered as party cookies.

2. Types of cookies as the length of time that remain active:

Depending on the length of time that remain active in the terminal equipment can be distinguished:

- Session cookies: cookies are a type designed to collect and store data while the user accesses a web page. Are usually used to store information that only worth preserving for the service requested by the user at any one time (eg a list of products purchased).

- Persistent cookies: cookies are a type of data which are stored in the terminal and can be accessed and treated for a period defined by the head of the cookie, and can range from a few minutes to several years.

3. Cookies types according to their purpose:

Depending on the purpose for which the data are processed through cookies, we can distinguish between:

- Technical cookies: these are those that allow the user to navigate through a web page or application platform and the use of different options or services it exist as, for example, control traffic and data communication, identify the session, access to restricted access parts, remember the elements of an order, make the buying process an order, make an application for registration or participation in an event, use security features while browsing store content for dissemination videos or sound or share content via social networks.

- Customization cookies: these are those that allow the user to access the service with some general characteristics based on a predefined set of criteria in the user terminal would eg language, the type of browser through which you access the service, the locale from which you access the service, etc.

- Analysis cookies: they are those that allow the responsible for them, monitoring and analyzing the behavior of users of the web sites that are linked. The information gathered through such cookies are used in measuring the activity of web sites, application or platform and for the profiling of user navigation of such sites, applications and platforms, in order to make improvements function data analysis how users use the service.

Management tool cookies

This Website uses Google Analytics.

Google Analytics is a free tool from Google that primarily allows website owners know how users interact with your website. Also, enable cookies in the domain of the site in which you are and uses a set of cookies called "__utma" and "__utmz" to collect information anonymously and reporting of website trends without identifying individual users..

For statistics of use of this website use cookies in order to know the level of recurrence of our visitors and more interesting content. This way we can concentrate our efforts on improving the most visited areas and make the user more easily find what they are looking for. On this site you can use the information from your visit for statistical evaluations and calculations anonymous data and to ensure the continuity of service or to make improvements to their websites. For more details, see the link below privacy policy [http://www.google.com/intl/en/policies/privacy/]

How to manage cookies on your computer: disabling and deleting cookies

All Internet browsers allow you to limit the behavior of a cookie or disable cookies within settings or browser settings. The steps for doing so are different for each browser, you can find instructions in the help menu of your browser.

If you decline the use of cookies, since it is possible thanks to the preferences menu of your browser or settings, reject, this website will continue to function properly without the use of the same.

Can you allow, block or delete cookies installed on your computer by setting your browser options installed on your computer:

- For more information about Internet Explorer click here.
- For more information on Chrome click here.
- For more information about Safari click here.
- For more information about Firefox click here.

Through your browser, you can also view the cookies that are on your computer, and delete them as you see fit. Cookies are text files, you can open and read the contents. The data within them is almost always encrypted with a numeric key corresponding to an Internet session so often has no meaning beyond the website who wrote it.

Informed consent

The use of this website on the other hand, implies that you paid your specific consent to the use of cookies, on the terms and conditions provided in this Cookies Policy, without prejudice to the measures of deactivation and removal of cookies that you can take, and mentioned in the previous section.