Molecular Environmental Microbiology Laboratory

deLorenzoVRed

 

Victor de Lorenzo

Group Leader

Contact

 

Research summary

Our laboratory is committed to understanding how bacteria that inhabit natural niches sense and process multiple environmental signals into distinct responses –both at the level of single cells and as a community. Unlike laboratory settings, in which growth conditions can be controlled and changed one at a time, bacteria in the environment must perpetually make decisions between activating metabolic genes for available, frequently mixed C-sources and those for escaping or adapting to physicochemical stress.

 

Publications

Dvořák P, Nikel PI, Damborský J, de Lorenzo V. Bioremediation 3.0: Engineering pollutant-removing bacteria in the times of systemic biology. Biotechnol Adv. 2017 Nov 15;35(7):845-866

Goñi-Moreno A, Benedetti I, Kim J, de Lorenzo V. Deconvolution of gene expression noise into spatial dynamics of transcription factor-promoter interplay. ACS Synth Biol 2017; 6: 1359-1369

Akkaya O, Pérez-Pantoja D, Calles B, Nikel PI, de Lorenzo, V. The metabolic redox regime of Pseudomonas putida tunes its evolvability towards novel xenobiotic substrates. mBio 2018; 9: e01512-18

Dvorak P, de Lorenzo V. Refactoring the upper sugar metabolism of Pseudomonas putida for co-utilization of cellobiose, xylose, and glucose. Metab Eng 2018; 19: e45658

de Lorenzo V. et al The power of synthetic biology for bioproduction, remediation and pollution control. EMBO Rep 2018; 6: e00340-1

Funding

logo ministerio eco com FEDER

 

 

 

Convocatoria Retos-Colaboración 2014, RTC-2014-1777-3: Desarrollo de herramientas metagenómicas para producción de biocombustibles (CAMBIOS).

 

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The longstanding mission our team is the production of biological agents for biosensing, remediation and (wherever possible) valorisation of chemical waste that is otherwise dumped into the Environment by urban and industrial activities. The workhorse to this end is the soil bacterium Pseudomonas putida, which combines the ease of genetic programming that is typical of Escherichia coli with the safety, robustness and metabolic capabilities required in whole-cell catalysts for applications in harsh biotechnological settings.

Specific activities include:

[i] Development of P. putida as a reliable chassis for implantation of genetic and metabolic circuits. This involves a profound editing of the extant genome of this microorganism for enhancing desirable properties and eliminating drawbacks. Also, the exploitation of surface-display systems for designing complex catalytic properties altogether separated from the cell metabolism and even the design of artificial communities by means of ectopic adhesins.

[ii] Genetic tools for deep refactoring of metabolic properties of P. putida. The list of new assets that we are developing includes a large collection of standardised plasmid and transposon vectors as well as dedicated reporter systems for parameterisation of the gene expression flow and for switching entire metabolic regimes.

[iii] The TOL system borne by plasmid pWW0 as a reference for metabolic circuit implantation. The two operons for toluene and m-xylene biodegradation encoded in pWW0 offer a natural case of expansion of the metabolic repertoire of environmental bacteria through acquisition of new genes.

[iv] Deep metabolic engineering of P. putida. Currents efforts attempt to develop strains that can be entirely programmed to deliver catalytic phenotypes of choice upon exposure and computation of both external and internal cues. This endeavour combines direct rational engineering with fine-tuning of gene expression by means of site-specific diversification of genomic sequences of choice through adaptation to P. putida of multiple automated genome engineering (MAGE) technology.

 

 

grupoDeLorenzo 

Name
Position
Contact
Víctor de Lorenzo Principal investigator
Belén Calles Senior Scientist
Esteban Martínez Postdoctoral scientist
Yamal Al-Ramahí Postdoctoral scientist
David Rodríguez Espeso Postdoctoral scientist
Tomás Aparicio Postdoctoral scientist
Mª de los Angeles Hueso Predoctoral scientist
Huseyin Tas Predoctoral scientist
Elena Velázquez Predoctoral scientist
Sofia Fraile Technicians
Elena Algar Technicians
Inés Merino Administrative assistant

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