Signalling networks in plant development and defense responses

JJSS

 RojoE 2021

José J. Sánchez Serrano

Group Leader

Contact

 

Enrique Rojo

Group Leader

Contact

 

 



Research summary

In our group we are interested in understanding how the progeny from stem cells decides between retaining pluripotency or initiating cell differentiation. We have characterized two genes in Arabidopsis, IYO and RIMA, which are core components of a switch activating cell differentiation in plants. We are now studying how this switch is regulated by developmental and extrinsic signals and what output of the switch is responsible for triggering differentiation. In addition to improving our understanding of plant development, our research could provide technology for targeting important traits such as organogenesis or regeneration capacity.

 

Publications

Chen L, Zhao M, Wu Z, Chen S, Enrique Rojo E, et al. RNA polymerase II associated proteins regulate stomatal development through directly interacting with the stomatal transcription factors in Arabidopsis thaliana. New Phytol. (2020).

Contreras R, Kallemi P, González-García MP, Lazarova A, Sánchez-Serrano JJ. Identification of domains and factors involved in MINIYO nuclear import. Front Plant Sci 2019; 10.

Delgadillo MO, Ruano G, Zouhar  J, Sauer  M, Shen J, et al. MTV proteins unveil ER- And microtubule-associated compartments in the plant vacuolar trafficking pathway. Proc Natl Acad Sci USA 2020; 117: 9884-9895.

Taurino, M., Costantini, S., De Domenico, S., Stefanelli, F., Ruano, G., Delgadillo, M.O., Sanchez-Serrano, J.J., Sanmartin, M., Santino, A., and Rojo, E. SEIPIN Proteins Mediate Lipid Droplet Biogenesis to Promote Pollen Transmission and Reduce Seed Dormancy. Plant Physiol 2018 176, 1531-1546.

Lynch, C.J., Bernad, R., Calvo, I., Nobrega-Pereira, S., Ruiz, S., Ibarz, N., Martinez-Val, A., Grana-Castro, O., Gomez-Lopez, G., Andres-Leon, E., Espinosa Angarica, V., Del Sol, A., Ortega, S., Fernandez-Capetillo, O., Rojo, E., Munoz, J., and Serrano, M. The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity. Cell Rep 2018, 22, 396-410.

Munoz, A., Mangano, S., Gonzalez-Garcia, M.P., Contreras, R., Sauer, M., De Rybel, B., Weijers, D., Sanchez-Serrano, J.J., Sanmartin, M., and Rojo, E. RIMA-Dependent Nuclear Accumulation of IYO Triggers Auxin-Irreversible Cell Differentiation in Arabidopsis. Plant Cell 2017 29, 575-588.

 

Our group studies how plants adjust their growth and development to changes in the environment, and, in particular, to challenges from pests and pathogens. These are some of the questions we are currently investigating:

1) How is stem cell differentiation initiated in plants? Our working hypothesis is that nuclear migration of the Arabidopsis proteins IYO and RIMA functions as a switch to reprogram the transcriptome and trigger stem cell differentiation. We are studying how IYO/RIMA nuclear localisation is controlled by developmental and environmental cues and how IYO and RIMA activate downstream targets to drive cell differentiation.

2) What are the mechanisms of vacuolar trafficking in plants ? Through a genetic screen, we are characterizing genes involved in protein transport to the vacuole and addressing the role of this organelle in plant development and defence.

3) Do non-vascular plants activate systemic wound responses and if so, how? Considering that in higher plants wound signals are transmitted through the vasculature, these studies could provide important clues on the development of systemic signalling systems during the evolution of land plants.

  Rojo SanchezSerrano fig1

VPS51 localizes to Microtubule-Associated Compartments. Max. intensity projection of serial confocal images (depth: 10 µm) of Nicotiana benthamiana epidermal cells co-transformed with pUBI:RFP-VPS51 and the microtubule marker GFP-MAP4. Scale bar: 10 µm.

 

316RojoGrupoBaja 

Name
Position
Contact
Jose J. Sánchez Serrano Principal investigator
Enrique Rojo Principal investigator
Maria Otilia Delgadillo Postdoctoral scientist
Aleksandra Lazarova Predoctoral scientist
Yolanda Fernández Technician

 

NOTE! This site uses cookies and similar technologies.

If you continue browsing or do not change browser settings, we consider your acepptance for using. Learn more

I understand

COOKIES POLICY

A cookie is a text file that is stored on your computer or mobile device via a web server and only that server will be able to retrieve or read the contents of the cookie and allow the Web site remember browser preferences and navigate efficiently. Cookies make the interaction between the user and the website faster and easier.

General information

This Website uses cookies. Cookies are small text files generated by the web pages you visit, which contain the session data that can be useful later in the website. In this way this Web remembers information about your visit, which can facilitate your next visit and make the website more useful.

How do cookies?

Cookies can only store text, usually always anonymous and encrypted. No personal information is ever stored in a cookie, or can be associated with identified or identifiable person.

The data allow this website to keep your information between the pages, and also to discuss how to interact with the website. Cookies are safe because they can only store information that is put there by the browser, which is information the user entered in the browser or included in the page request. You can not run the code and can not be used to access your computer. If a website encrypts cookie data, only the website can read the information.

What types of cookies used?

The cookies used by this website can be distinguished by the following criteria:

1. Types of cookies as the entity that manages:

Depending on who the entity operating the computer or domain where cookies are sent and treat the data obtained, we can distinguish:

- Own cookies: are those that are sent to the user's terminal equipment from a computer or domain managed by the editor itself and from which provides the service requested by the user.

- Third party cookies: these are those that are sent to the user's terminal equipment from a machine or domain that is not managed by the publisher, but by another entity data is obtained through cookies.

In the event that the cookies are installed from a computer or domain managed by the editor itself but the information collected by these is managed by a third party can not be considered as party cookies.

2. Types of cookies as the length of time that remain active:

Depending on the length of time that remain active in the terminal equipment can be distinguished:

- Session cookies: cookies are a type designed to collect and store data while the user accesses a web page. Are usually used to store information that only worth preserving for the service requested by the user at any one time (eg a list of products purchased).

- Persistent cookies: cookies are a type of data which are stored in the terminal and can be accessed and treated for a period defined by the head of the cookie, and can range from a few minutes to several years.

3. Cookies types according to their purpose:

Depending on the purpose for which the data are processed through cookies, we can distinguish between:

- Technical cookies: these are those that allow the user to navigate through a web page or application platform and the use of different options or services it exist as, for example, control traffic and data communication, identify the session, access to restricted access parts, remember the elements of an order, make the buying process an order, make an application for registration or participation in an event, use security features while browsing store content for dissemination videos or sound or share content via social networks.

- Customization cookies: these are those that allow the user to access the service with some general characteristics based on a predefined set of criteria in the user terminal would eg language, the type of browser through which you access the service, the locale from which you access the service, etc.

- Analysis cookies: they are those that allow the responsible for them, monitoring and analyzing the behavior of users of the web sites that are linked. The information gathered through such cookies are used in measuring the activity of web sites, application or platform and for the profiling of user navigation of such sites, applications and platforms, in order to make improvements function data analysis how users use the service.

Management tool cookies

This Website uses Google Analytics.

Google Analytics is a free tool from Google that primarily allows website owners know how users interact with your website. Also, enable cookies in the domain of the site in which you are and uses a set of cookies called "__utma" and "__utmz" to collect information anonymously and reporting of website trends without identifying individual users..

For statistics of use of this website use cookies in order to know the level of recurrence of our visitors and more interesting content. This way we can concentrate our efforts on improving the most visited areas and make the user more easily find what they are looking for. On this site you can use the information from your visit for statistical evaluations and calculations anonymous data and to ensure the continuity of service or to make improvements to their websites. For more details, see the link below privacy policy [http://www.google.com/intl/en/policies/privacy/]

How to manage cookies on your computer: disabling and deleting cookies

All Internet browsers allow you to limit the behavior of a cookie or disable cookies within settings or browser settings. The steps for doing so are different for each browser, you can find instructions in the help menu of your browser.

If you decline the use of cookies, since it is possible thanks to the preferences menu of your browser or settings, reject, this website will continue to function properly without the use of the same.

Can you allow, block or delete cookies installed on your computer by setting your browser options installed on your computer:

- For more information about Internet Explorer click here.
- For more information on Chrome click here.
- For more information about Safari click here.
- For more information about Firefox click here.

Through your browser, you can also view the cookies that are on your computer, and delete them as you see fit. Cookies are text files, you can open and read the contents. The data within them is almost always encrypted with a numeric key corresponding to an Internet session so often has no meaning beyond the website who wrote it.

Informed consent

The use of this website on the other hand, implies that you paid your specific consent to the use of cookies, on the terms and conditions provided in this Cookies Policy, without prejudice to the measures of deactivation and removal of cookies that you can take, and mentioned in the previous section.