Proteomics facility



Fernando Corrales

Head of service





Service Description

figureProteomicsCreated in 1999, the CNB Proteomics Facility maintains a technological platform suitable for large-scale protein identification and characterisation, offering its services to the CNB scientific community as well as to external researchers. Massive protein identification and characterisation is performed by multidimensional nano-HPLC chromatography coupled to a nano-electrospray ion trap mass spectrometer (MS), to a TripleQ-TOF MS, or to a MALDI TOF/TOF MS (LC-MS/MS). For differential proteomics (quantitative proteomics), we use metabolic or chemical stable isotope labelling (ICPL, SILAC, iTRAQ,TMT) combined with LC-MS/MS analysis. We also offer targeted and, in combination with AQUA peptides, absolute quantitative protein analysis by selected/multiple reaction monitoring (S/MRM-MS). Phosphorylation analysis is performed through specific phosphopeptide enrichment procedures followed by LC-MS/MS analysis, using CID (collision-induced dissociation) or ETD (electron transfer dissociation) fragmentation methods. Prolamin detection and characterisation by ELISA and mass spectrometry are also offered in our analysis portfolio. For educational purposes, we organise practical courses on topics such as quantitative proteomics and bioinformatics. The CNB Proteomics Facility is a member of the Proteored-ISCIII Platform (Plataforma en Red de Proteómica-Carlos III).



  • Two-dimensional gel electrophoresis
  • Protein identification and characterisation by MALDI-TOF/TOF, ESI Q-TOF, ESI QQQ and ESI ion trap mass spectrometry
  • Protein quantitation by metabolic and chemical stable isotopic labelling (SILAC, ICPL, iTRAQ, TMT)
  • Selected/multiple reaction monitoring (S/MRM-MS)
  • Identification and characterisation of post-translational modifications
  • Peptide synthesis and membrane-bound peptide array design
  • Gluten analysis by ELISA and mass spectrometry


Fees and others


Fernado Corrales Head of service
Alberto Paradela Personnel
Adan Alpizar Personnel
Sergio Ciordia Personnel
Marta García Personnel
Manuel Lombardía Personnel
Miguel Marcilla Personnel
Rosana Navajas Postdoctoral Scientist
Lorena Carmona Personnel
Antonio Ramos Proteobotics Data Analyser

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