Flow cytometry



Mª del Carmen Moreno-Ortiz Navarro

Group Leader







Service Description

cell proliferationFlow cytometry (CMF) is a technology of multiparametric cell analysis for detection and identification of molecules and cell structures using fluorescent markers and conjugated antibodies. Flow sorting is an extension of this technology, by which any cell or object measured can be separated selectively from the suspension based on properties measured in the flow stream. Flow cytometry techniques have undergone significant development in recent years in their ability to analyse thousands of cells per second and provide statistical information instantly.

The Flow Cytometry Unit provides scientific and technological support to CNB research groups,as well as to researchers from the public and private sectors.


The unit offers

  • Training and advice on flow cytometry principles and applications
  • Development of new applications and experimental design, incorporating new technologies and reagents
  • Quantification of secreted cytokines by multiplex assays
  • Professional analysis with specific software
  • Cellular isolation by cell sorting
  • Maintenance of stock of antibodies and commonly used reagents


  • BD FACSCalibur Analyzer: 4 colours, 2 laser (488 nm and 633 nm)
  • Beckman Coulter EPICS XL-MCL Analyzer: 4 colours, 1 laser (488 nm)
  • Beckman Coulter CYTOMICS FC 500 Analyzer: 5 colours, 2 laser (488 nm and 633 nm)
  • Beckman Coulter CYTOMICS FC 500 Analyzer: 5 colours, 1 laser (488 nm)
  • BD LSRII Analyzer: 8 colors, 3 laser (488 nm, 633 nm and 405 nm)
  • Beckman Coulter GALLIOS Analyzer: 10 colours, 3 laser (488 nm, 633 nm and 405 nm)
  • Luminex 100 IS Multiparametric Analyzer: A system that can be used to quantify multiple cytokines (up to 100) or any other soluble molecule from a single sample
  • Cell Sorter Beckman Coulter Moflow XDP: 10 colours, 3 laser (488 nm, 633 nm and 405 nm)
  • The unit also provides 3 PC platforms for analysis with specific software: WindMDI, CXP, MultiTime, MultiCycle, DIVA, FlowJo, Summit, and Kaluza

Common applications performed routinely:Ploidy levels in plants IP

  • Cell viability (IP, 7AAD, DAPI)
  • Viability fixed cells; cell cycle and ploidy (IP, DAPI)
  • Studies of mitotic populations (G2/M) with phospho histone 3
  • Ploidy levels in plants (IP)
  • Proliferation assessment with BrdU o EdU)
  • Cell proliferation (CFSE, CellTrace)
  • Apoptosis (SubG0/G1, annexin V, TUNEL)
  • Gene expression using fluorescent proteins
  • Intracellular signalling (phosphoproteins)
  • Cell migration studies
  • Intracellular Ca2+ mobilisation
  • Immunophenophenotyping using multiparametric analysis up to 10 colours
  • Study of T regulatory cells; intracellular cytokines
  • Quantitation of soluble molecules, cytokines using multiplex assays
  • Cell sorting


Fees and others


Datos adjuntos sin título 00026Red 

Mª del Carmen Moreno-Ortiz Navarro Lead scientist
Sara Escudero García Personnel

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