Research
Towards extending the use of Electron Microscopy at different structural levels
The activity of the group aims to extend the use of improved methods in three-dimensional cryo-electron microscopy (3D-cryo-EM) in two main directions: Towards reaching subnanometer resolution using single particle reconstruction approaches, and towards the analysis of the subcellular domain using electron tomography
Molecular resolutionThe model system we study is the assembly and maturation process of complex double stranded DNA viruses. We use the phages T7 and phi29 as model systems offering complementary aspects to cover the most interesting features shared by this virus family during their assembly, as the production of intermediates, the expansion of the shell by a complex subunit rearrangement, the transformation of inner capsid components (core, scaffolding), and the changes involved in the function of the DNA packaging machinery. We also improve the single particle reconstruction methods to get higher resolution data suitable to apply hybrid approaches, by a combination of 3D-cryo-EM and modelling methods, to compare quasi-atomic 3D maps of proheads and mature heads of T7. |
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Electron TomographyThe interactions of virus with the target cells are studied using electron tomography of infected cells. We are involved in the development of Tomographic methods using model large viruses (as Poxviruses) as test objects combining correlation with single particle reconstruction approaches. We are also participating in the structural characterisation of the centrosome, in close collaboration with the group of Jose M. Valpuesta. Furthermore, our group is working in the possibility to correlate these methods with novel X-ray microscopy attempts. To this end, we are part of the group that is setting up a dedicated beam line for this purpose in the Spanish Synchrotron ALBA. |
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Correlation of structural data and physical properties at the single particle level
The structural data obtained by 3D-cryo-EM from the different experimental systems under study in our group are currently combined with the study of the nano-mechanical properties of individual viral particles using atomic force microscopy and spectroscopy. We are also exploring the application of optical tweezers to test small forces involved in specific capsid transformations. The study of the deformation behaviour and material properties at the level of individual viral particles opens the possibility to perform systematic studies to correlate molecular structure, nanoscopic behaviour, and macroscopic properties of these macromolecular viral containers.
Members
Jaime Martín-Benito
Ricardo Arias
Post-doctoral investigators
Fco Javier Chichón
Borja Ibarra
Ana Cuervo
Predoctoral students
Michele Chiappi
María Ibarra Daudén
Alina Elena Ionel
Veronica Gonzalez García
Technician
Maria José Rodriguez
Mar Pulido
Encarna Cebrián
Publications
2011
Hormeño S, Ibarra B, Valpuesta JM, Carrascosa JL, Arias-Gonzalez JR. Mechanical stability of low-humidity single DNA molecules. Biopolymers. 2011 Oct 23. doi: 10.1002/bip.21728. [Epub ahead of print].PMID:22020764[PubMed - as supplied by publisher]
Irena L. Ivanovska, Roberto Miranda, J.L. Carrascosa, Gijs J.L. Wuite and Christoph F. Schmidt.
Discrete fracture patterns of virus shells reveal mechanical building blocks.
Proc. Natl. Acad. Sci. USA. 108 (31), 12611-12616 (2011).
Silvia Hormeño, Fernando Moreno-Herrero, Borja Ibarra, J.L. Carrascosa, José M. Valpuesta, and J. Ricardo Arias-Gonzalez.
Condensation Prevails over B-A Transition in the Structure of DNA at Low Humidity.
Biophysical Journal, 100, 2006-2015 (2011).
Silvia Hormeño, Borja Ibarra, J.L. Carrascosa, José M. Valpuesta, Fernando Moreno-Herrero and J. Ricardo Arias-Gonzalez.
Mechanical Properties of High-G•C Content DNA with A-Type Base-Stacking.
Biophysical Journal, 100, 1996-2005 (2011).
C. Carrasco, A. Luque, M. Hernando-Pérez, R. Miranda, J.L. Carrascosa, P.A. Serena, M. de Ridder, A. Raman, J. Gómez-Herrero, I.A.T. Schaap, D. Reguera and P.J. de Pablo.
Built-In Mechanical Stress in Viral Shells.
Biophysical Journal, 100, 1100-1108 (2011).
Alina Ionel, Javier A. Velazquez-Muriel, Daniel Luque, Ana Cuervo, José R. Caston, José M. Valpuesta, Jaime Martin-Benito and José L. Carrascosa.
Molecular Rearrangements Involved in the Capsid Shell Maturation of Bacteriophage T7.
J Biol Chem., 286 (1) 234-242 (2011).
2010
Ignacio Arechaga, Oscar H. Martínez-Costa, Cristina Ferreras, José L. Carrascosa, and Juan J. Aragón.
Electron microscopy analysis of mammalian phosphofructokinase reveals an unusual 3-dimensional structure with significant implications for enzyme function.
The FASEB Journal. 24, 4960-4968 (2010)
Luque D, González JM, Garriga D, Ghabrial SA, Havens WM, Trus B, Verdaguer N, Carrascosa JL, Castón JR.
The T=1 capsid protein of Penicillium chrysogenum virus is formed by a repeated helix-rich core indicative of gene duplication.
J Virol. 84 (14) 7256-7266 (2010).
Saugar I, Irigoyen N, Luque D, Carrascosa JL, Rodríguez JF, Castón JR. Electrostatic interactions between capsid and scaffolding proteins mediate the structural polymorphism of a double-stranded RNA virus.
J Biol Chem. 285 (6). 3643-50, 2010
Jason Pierson, José Jesús Fernández, Erik Bos, Shoaib Amini, Helmut Gnaegi, Matthijn Vos, Bennie Bel, Freek Adolfsen, José L. Carrascosa and Peter J. Peters. (2010)
Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: Electrostatic charging for section attachment and implementation of an anti-contamination glove box.
Journal of Structural Biology. 169, 219-225.
2009
P.A. Serena, M. Douas, M.I. Marqués, C. Carrasco, P.J. de Pablo, R. Miranda, J.L. Carrascosa, M. Castellanos and M.G. Mateu. (2009) MC simulations of wáter meniscus in nanocontainers: explaining the collapse of viral particles due to capillary forces.Phys. Status Solidi C, 6, nº10, 2128-2132.
César Reiriz, Roberto J. Brea, Rocío Arranz, José L. Carrascosa, Alejandra Garibotti, Brendan Manning, José M. Valpuesta, Ramón Eritja, Luis Castedo and Juan R. Granja. (2009) α,γ-Peptide Nanotube Templating of One-Dimensional Parallel Fullerene Arrangements.J. American Chemical Society, 131, 11335-11337.
John Melcher, Carolina Carrasco, Xin Xu, José L. Carrascosa, Julio Gómez-Herrero, Pedro José de Pablo and Arvind Raman. (2009) Origins of phase contrast in the atomic force microscope in liquids.
Proc. Natl. Acad. Sci. USA., 106, nº 33, 13655-13660.
José L. Carrascosa, Francisco Javier Chichón, Eva Pereiro, María Josefa Rodríguez, Jose Jesús Fernández, Mariano Esteban, Stefan Heim, Peter Guttmann, Gerd Schneider. (2009) Cryo-X-ray tomography of vaccinia virus membranes and inner compartments. Journal of Structural Biology. 168, 234-239.
Paloma Gutiérrez del Arroyo, Marisela Vélez, Oliver Piétrement, Margarita Salas, José L.Carrascosa, Ana Camacho. (2009) A nucleoprotein-hairpin in transcription regulation. Journal of Structural Biology, 168, 444-451.
S. Hormeño, B. Ibarra, F.J. Chichón, K. Habermann, B.M.H. Lange, J.M. Valpuesta, J.L. Carrascosa and J.R. Arias-González. (2009) Single Centrosome Manipulation Reveals Its Electric Charge and Associated Dynamic Structure.Biophysical Journal, 97, 1022-1030.
Rocío Coloma, José M. Valpuesta, Rocío Arranz, José L. Carrascosa, Juan Ortín, Jaime Martín-Benito. (2009) The structure of a Biologically Active Influenza Virus Ribonucleoprotein Complex.PLOS Pathogens, 5, Issue 6, e100049.
Carrasco, C., Douas, M., Miranda, R., Castellanos, M., Serena, P.A., Carrascosa, J.L., Mateu, M.G., Marqués, M.I., and de Pablo, P.J. (2009) The capillarity of nanometric water menisci confined incide closed-geometry viral cages.Proc. Natl. Acad. Sci. USA., 106, 2148-2152.
Luque D, Rivas G, Alfonso C, Carrascosa JL, Rodríguez JF, Castón JR. (2009) Infectious bursal disease virus is an icosahedral polyploid dsRNA virus. Proc. Natl. Acad. Sci. USA., 106, 2148-2152.
Chichón FJ, Rodríguez MJ, Risco C, Fraile-Ramos A, Fernández JJ, Esteban M, Carrascosa JL. (2009) Membrane remodeling during vaccinia virus morphogenesis. Biol. Cell., 101, 401-404.
Luque D, Saugar I, Rejas MT, Carrascosa JL, Rodríguez JF, Castón JR. (2009) Infectious Bursal Disease Virus: Ribonucleoprotein Complexes of a Double-Stranded RNA Virus. J. Mol. Biol., 386, 891-901.
2008
J.J. Fernández, D. Luque, J.R. Castón, José L. Carrascosa. (2008) Sharpening high resolution information in single particle electron cryomicroscopy. Journal of Structural Biology, 164 pp, 170-175.
Cuellar, J., Martin-Benito, J., Scheres, SHW., Sousa, R., Moro, F., Lopez-Vinas, E., Gomez-Puertas, P., Muga A., Carrascosa, J.L., Valpuesta, J.M. (2008) The structure of CCT-Hsc70(NBD) suggests a mechanism for Hsp70 delivery of substrates to the chaperonin. Nature Structural & Molecular Biology, 15, Issue: 8, 858-864.
Fábio Fernández, Luís M.S. Loura, Francisco J. Chichón, José L. Carrascosa, Alexander Fedorox y Manuel Prieto. (2008). Role Of Helix-0 of the N-BAR Domain in Membrane Curvature Generation. Biophysical Journal, 94, 3065-3073.
2007
Luque,D., Saugar,I., Rodriguez,J.F., Verdaguer,N., Garriga,D., Martin,C.S., Velazquez-Muriel,J.A., Trus,B.L., Carrascosa,J.L., and Caston,J.R. (2007). Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch. J. Virol., 81, 6869-6878.
Agirrezabala,X., Velazquez-Muriel,J.A., Gomez-Puertas,P., Scheres,S.H.W., Carazo,J.M., and Carrascosa,J.L. (2007). Quasi-atomic model of bacteriophage T7 procapsid shell: Insights into the structure and evolution of a basic fold. Structure, 15, 461-472.
Jaime Martín-Benito, Julie Grantham, Jasminka Boskovic, Karen I. Brackley, José L. Carrascosa, Keith R. Willison y José M. Valpuesta. (2007). The inter-ring arrangement of the cytosolic chaperonin CCT. EMBO Reports. 8 (3), 252-257.
Ivanovska IL , Agirrezabala X, Carrascosa JL, Schmidt CF, Wuite GJL. (2007). Deconstructing viral capsids reveals their strengths and waknesses. Biophysical Journal : 49A Suppl.
2006
Georgia Kapatai, Andrew Large, Justin. L. P. Benesch, Carol V. Robinson, José L. Carrascosa, Jose M. Valpuesta, Preethy Gowrinathan and Peter A. Lund. (2006). All three chaperonin genes in the archaeon Haloferax volcanii are individually dispensable.
Molecular Microbiology 61 (6). 1583-1597.
José R. Castón, Daniel Luque, Benes L. Trus, Germán Rivas , Carlos Alfonso, José M. González, José L. Carrascosa, Padmanaban Annamalai, Said A. Ghabrial. (2006). Tree-dimensional structure and stoichiometry of Helmintosporium victoriae190S totivirus. Virology 347. 323-332.
2005
Agirrezabala,X., Martin-Benito,J., Caston,J.R., Miranda,R., Valpuesta,J.M., and Carrascosa,J.L. (2005). Maturation of phage T7 involves structural modification of both shell and inner core components. EMBO J., 24, 3820-3829.
Agirrezabala,X., Martin-Benito,J., Valle,M., Gonzalez,J.M., Valencia ,A., Valpuesta,J.M., and Carrascosa,J.L. (2005). Structure of the connector of bacteriophage T7 at 8A resolution: structural homologies of a basic component of a DNA translocating machinery. J Mol Biol, 347, 895-902.
Cyrklaff,M., Risco,C., Fernandez,J.J., Jimenez,M.V., Esteban,M., Baumeister,W., and Carrascosa,J.L. (2005). Cryo-electron tomography of vaccinia virus. Proc. Natl. Acad. Sci. U. S. A, 102, 2772-2777.
Saugar, I. , Luque,D., Ona,A., Rodriguez,J.F., Carrascosa,J.L., Trus,B.L., and Caston,J.R. (2005). Structural polymorphism of the major capsid protein of a double-stranded RNA virus: an amphipathic alpha helix as a molecular switch. Structure., 13, 1007-1017.
Bertrand,S.; Barthelemy, I. ; Oliva,M.A.; Carrascosa,J.L.; Andreu,J.M.; Valpuesta,J.M. (2005). Folding, stability and polymerization properties of FtsZ chimeras with inserted tubulin loops involved in the interaction with the cytosolic chaperonin CCT and in microtubule formation . J. Mol. Biol . Vol. 346 . pp. 319-330.
J. Querol-Audi, R. Pérez-Luque, I. Fita, C. López-Iglesias, J.R. Castón, J.L. Carrascosa and N. Verdaguer (2005). Preliminary analysis of two and three-dimensional crystals of vault ribonucleoprotein particles. J. Struct. Biol . Vol. 151 , pp. 111-115.
2004
I. L. Ivanovska, P. J. de Pablo, B. Ibarra, G. Sgalari, F. C. MacKintosh, J. L. Carrascosa, C. F. Schmidt, and G. J. L. Wuite (2004). Bacteriophage capsids: Tough nanoshells with complex elastic properties. Proc. Natl. Acad. Sci. USA . 101 , 7600-7605..
Barcena,J.; Verdaguer,N.; Roca,R.; Morales,M.; Angulo,I.; Risco,C.; Carrascosa,J.L.; Torres,J.M.; Caston,J.R. (2004). The coat protein of Rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid. Virology 322 , 118-134.
J. Martín-Benito, S. Bertrand, T. Hu, P. J. Ludtke, J. N. McLaughlin, B. M. Willardson, J. L. Carrascosa and J. M. Valpuesta. (2004). Structure of the complex between the cytosolic chaperonin CCT and phosducin-like protein. Proc. Natl. Acad. Sci. USA , 101, 17410-17415.
P. Gómez-Puertas, J. Martín-Benito, J. L. Carrascosa, K. R. Willison and J. M. Valpuesta (2004). The substrate recognition mechanisms in chaperonins. J. Mol. Recognit . 17 . 85- 94.
Area, E.; Martin-Benito, J.; Gastaminza, P.; Torreira, E.; Valpuesta, J.M.; Carrascosa, J.L.; Ortin, J. (2004). 3D structure of the influenza virus polymerase complex: localization of subunit domains. Proc. Natl. Acad. Sci. USA . 101 . 308- 313.
2003
IJ Salanueva, RR Novoa, P Cabezas, C LopezIglesias, JL Carrascosa, RM Elliott, C Risco (2003). Polymorphi sm and structural maturation of Bunyamwera virus in golgi and post-golgi compartments. Journal of Virology 77 , Iss 2, pp 1368-1381.
J.R. Castón, S.A. Gabrial, D. Jiang, G. Rivas, C. Alfonso, R. Roca, D. Luque and J.L. Carrascosa (2003). Three-dimensional structure of Penicillium chrysogenum virus: A double-strnded RNA virus with a genuine T=1 capsid. J. Mol. Biol . 331 , 417-431.
J.C. Gallego-Gómez, C. Risco, D. Rodríguez, P- Cabezas, S. Guerra, J.L. Carrascosa and M. Esteban. (2003). Differences in virus-induced cell morphology and in virus maturation between MVA and other strains (WR, Ankara and NYCBH) of vaccinia virus in infected human cells. J. Virol . 77 , 10606-10622.
C. Risco, J.L. Carrascosa and T. Frey. (2003). Structural maturation of rubella virus in the Golgi complex. Virology 312 , pp 261-269.
Area,E.; Martin-Benito,J.; Gastaminza,P.; Torreira,E.; Valpuesta,J.M.; Carrascosa,J.L.; Ortin,J. (2004) .3D structure of the influenza virus polymerase complex: localization of subunit domains. Proc. Natl. Acad. Sci. USA . 101 , 308-313.
P. Gómez-Puertas, J. Martín-Benito, J. L. Carrascosa, K. R. Willison and J. M. Valpuesta (2004). The substrate recognition mechanisms in chaperonins. J. Mol. Recognit . 17 , 85-94.
Funding
Active Funding
2012-2014
Analisis estructural integrado a distintos niveles de resolución: Estudio de sistemas virales modelo.
BFU2011-29038-C02-01.
I.P.: J.L. Carrascosa.
2011-2015
Transnational access and enhancement of integrated Biological Structure determination at Synchrotron X-ray radiation facilities.
FP7-Infrastructures-2011-1: 283570 BioStruct-X
Participant: J.L. Carrascosa.
2011-2015.
Multifuncional nanotechnology for selective detection and treatment of cáncer.
FP7-NMP-2010-Large-4: 262943-2 MULTIFUN
C-NMP/0878 European Union. CSIC.
José L. Carrascosa.
2010-2014.
Nuevos materiales y dispositivos biofuncionales híbridos en Nanociencia. NOBIMAT-M.
S2009MAT-1507. Programa I+D de la Comunidad de Madrid en Tecnologías 2009. CSIC.
José L. Carrascosa (9).
2009-2011.
Soporte al Comité Nacional de la IUPAB.
ACI2008-0805. Programa Nacional de Internacionalización I + D. MCIN.
José L. Carrascosa (6).
2008-2012.
Consolider-Ingenio 2010: Nanociencia Molecular.
CSD2007-00010
José L. Carrascosa (10).
2009-2011.
Análisis de las bases moleculares del proceso de ensamblaje y maduración de partículas virales.
BFU2008-02328/BMC
José L. Carrascosa (10).
2006-2009
Synthetic Biomimetic Nanoengines: A modular Platform for Engineering of Nanomechamical Actuator Building blocks. (NANOMOT).
UE; 6 FP; NEST-029084. CSIC
José L. Carrascosa (6).
2006-2009
Análisis de estructura y función de nanomáquinas y otros sistemas biológicos y su integración mediante técnicas de manipulación de moléculas individuales.
CAM. S-0505/MAT/0283-05. CSIC
José L. Carrascosa (10).
2006-2009
A multidisciplinary approoach to determine the structures of protein complexes in a model organism. 3D-Repertoire.
BFU2006-26086E. Programa Nacional de Biología Fundamental. MEC. CSIC
José L. Carrascosa.
2005-2009.
A multidisciplinary approoach to determine the structures of protein complexes in a model organism.
3D-Repertoire.
LSHG-CT-2005-512028. European Union. Framework Program 6. CSIC
José L. Carrascosa (18).
2004-2009
New electron microscopy approaches for studying protein complexes and cellular supramolecular architecture.Acronym: 3D-EM.
LifeSci/NoE/FP6-502828. European Union, Framework Program 6. CSIC
José L. Carrascosa (15).
Resources
| The structural data obtained by 3D-cryo-EM from the different experimental systems under study in our group are currently combined with the study of the nano-mechanical properties of individual viral particles using atomic force microscopy and spectroscopy. We are also exploring the application of optical tweezers to test small forces involved in specific capsid transformations. The study of the deformation behaviour and material properties at the level of individual viral particles opens the possibility to perform systematic studies to correlate molecular structure, nanoscopic behaviour, and macroscopic properties of these macromolecular viral containers. |
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Members
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Jaime Martín-Benito |
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Ricardo Arias |
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Post-doctoral investigators |
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Fco Javier Chichón |
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Borja Ibarra |
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Ana Cuervo |
Predoctoral students |
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Michele Chiappi |
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María Ibarra Daudén |
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Alina Elena Ionel |
Veronica Gonzalez García |
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Technician |
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Maria José Rodriguez |
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Mar Pulido |
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Encarna Cebrián |
2011
Hormeño S, Ibarra B, Valpuesta JM, Carrascosa JL, Arias-Gonzalez JR. Mechanical stability of low-humidity single DNA molecules. Biopolymers. 2011 Oct 23. doi: 10.1002/bip.21728. [Epub ahead of print].PMID:22020764[PubMed - as supplied by publisher]
Irena L. Ivanovska, Roberto Miranda, J.L. Carrascosa, Gijs J.L. Wuite and Christoph F. Schmidt.
Discrete fracture patterns of virus shells reveal mechanical building blocks.
Proc. Natl. Acad. Sci. USA. 108 (31), 12611-12616 (2011).
Silvia Hormeño, Fernando Moreno-Herrero, Borja Ibarra, J.L. Carrascosa, José M. Valpuesta, and J. Ricardo Arias-Gonzalez.
Condensation Prevails over B-A Transition in the Structure of DNA at Low Humidity.
Biophysical Journal, 100, 2006-2015 (2011).
Silvia Hormeño, Borja Ibarra, J.L. Carrascosa, José M. Valpuesta, Fernando Moreno-Herrero and J. Ricardo Arias-Gonzalez.
Mechanical Properties of High-G•C Content DNA with A-Type Base-Stacking.
Biophysical Journal, 100, 1996-2005 (2011).
C. Carrasco, A. Luque, M. Hernando-Pérez, R. Miranda, J.L. Carrascosa, P.A. Serena, M. de Ridder, A. Raman, J. Gómez-Herrero, I.A.T. Schaap, D. Reguera and P.J. de Pablo.
Built-In Mechanical Stress in Viral Shells.
Biophysical Journal, 100, 1100-1108 (2011).
Alina Ionel, Javier A. Velazquez-Muriel, Daniel Luque, Ana Cuervo, José R. Caston, José M. Valpuesta, Jaime Martin-Benito and José L. Carrascosa.
Molecular Rearrangements Involved in the Capsid Shell Maturation of Bacteriophage T7.
J Biol Chem., 286 (1) 234-242 (2011).
2010
Ignacio Arechaga, Oscar H. Martínez-Costa, Cristina Ferreras, José L. Carrascosa, and Juan J. Aragón.
Electron microscopy analysis of mammalian phosphofructokinase reveals an unusual 3-dimensional structure with significant implications for enzyme function.
The FASEB Journal. 24, 4960-4968 (2010)
Luque D, González JM, Garriga D, Ghabrial SA, Havens WM, Trus B, Verdaguer N, Carrascosa JL, Castón JR.
The T=1 capsid protein of Penicillium chrysogenum virus is formed by a repeated helix-rich core indicative of gene duplication.
J Virol. 84 (14) 7256-7266 (2010).
Saugar I, Irigoyen N, Luque D, Carrascosa JL, Rodríguez JF, Castón JR. Electrostatic interactions between capsid and scaffolding proteins mediate the structural polymorphism of a double-stranded RNA virus. J Biol Chem. 285 (6). 3643-50, 2010
Jason Pierson, José Jesús Fernández, Erik Bos, Shoaib Amini, Helmut Gnaegi, Matthijn Vos, Bennie Bel, Freek Adolfsen, José L. Carrascosa and Peter J. Peters. (2010) Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: Electrostatic charging for section attachment and implementation of an anti-contamination glove box. Journal of Structural Biology. 169, 219-225.
2009
P.A. Serena, M. Douas, M.I. Marqués, C. Carrasco, P.J. de Pablo, R. Miranda, J.L. Carrascosa, M. Castellanos and M.G. Mateu. (2009) MC simulations of wáter meniscus in nanocontainers: explaining the collapse of viral particles due to capillary forces.Phys. Status Solidi C, 6, nº10, 2128-2132.
César Reiriz, Roberto J. Brea, Rocío Arranz, José L. Carrascosa, Alejandra Garibotti, Brendan Manning, José M. Valpuesta, Ramón Eritja, Luis Castedo and Juan R. Granja. (2009) α,γ-Peptide Nanotube Templating of One-Dimensional Parallel Fullerene Arrangements.J. American Chemical Society, 131, 11335-11337.
John Melcher, Carolina Carrasco, Xin Xu, José L. Carrascosa, Julio Gómez-Herrero, Pedro José de Pablo and Arvind Raman. (2009) Origins of phase contrast in the atomic force microscope in liquids.
Proc. Natl. Acad. Sci. USA., 106, nº 33, 13655-13660.
José L. Carrascosa, Francisco Javier Chichón, Eva Pereiro, María Josefa Rodríguez, Jose Jesús Fernández, Mariano Esteban, Stefan Heim, Peter Guttmann, Gerd Schneider. (2009) Cryo-X-ray tomography of vaccinia virus membranes and inner compartments. Journal of Structural Biology. 168, 234-239.
Paloma Gutiérrez del Arroyo, Marisela Vélez, Oliver Piétrement, Margarita Salas, José L.Carrascosa, Ana Camacho. (2009) A nucleoprotein-hairpin in transcription regulation. Journal of Structural Biology, 168, 444-451.
S. Hormeño, B. Ibarra, F.J. Chichón, K. Habermann, B.M.H. Lange, J.M. Valpuesta, J.L. Carrascosa and J.R. Arias-González. (2009) Single Centrosome Manipulation Reveals Its Electric Charge and Associated Dynamic Structure.Biophysical Journal, 97, 1022-1030.
Rocío Coloma, José M. Valpuesta, Rocío Arranz, José L. Carrascosa, Juan Ortín, Jaime Martín-Benito. (2009) The structure of a Biologically Active Influenza Virus Ribonucleoprotein Complex.PLOS Pathogens, 5, Issue 6, e100049.
Carrasco, C., Douas, M., Miranda, R., Castellanos, M., Serena, P.A., Carrascosa, J.L., Mateu, M.G., Marqués, M.I., and de Pablo, P.J. (2009) The capillarity of nanometric water menisci confined incide closed-geometry viral cages.Proc. Natl. Acad. Sci. USA., 106, 2148-2152.
Luque D, Rivas G, Alfonso C, Carrascosa JL, Rodríguez JF, Castón JR. (2009) Infectious bursal disease virus is an icosahedral polyploid dsRNA virus. Proc. Natl. Acad. Sci. USA., 106, 2148-2152.
Chichón FJ, Rodríguez MJ, Risco C, Fraile-Ramos A, Fernández JJ, Esteban M, Carrascosa JL. (2009) Membrane remodeling during vaccinia virus morphogenesis. Biol. Cell., 101, 401-404.
Luque D, Saugar I, Rejas MT, Carrascosa JL, Rodríguez JF, Castón JR. (2009) Infectious Bursal Disease Virus: Ribonucleoprotein Complexes of a Double-Stranded RNA Virus. J. Mol. Biol., 386, 891-901.
2008
J.J. Fernández, D. Luque, J.R. Castón, José L. Carrascosa. (2008) Sharpening high resolution information in single particle electron cryomicroscopy. Journal of Structural Biology, 164 pp, 170-175.
Cuellar, J., Martin-Benito, J., Scheres, SHW., Sousa, R., Moro, F., Lopez-Vinas, E., Gomez-Puertas, P., Muga A., Carrascosa, J.L., Valpuesta, J.M. (2008) The structure of CCT-Hsc70(NBD) suggests a mechanism for Hsp70 delivery of substrates to the chaperonin. Nature Structural & Molecular Biology, 15, Issue: 8, 858-864.
Fábio Fernández, Luís M.S. Loura, Francisco J. Chichón, José L. Carrascosa, Alexander Fedorox y Manuel Prieto. (2008). Role Of Helix-0 of the N-BAR Domain in Membrane Curvature Generation. Biophysical Journal, 94, 3065-3073.
2007
Luque,D., Saugar,I., Rodriguez,J.F., Verdaguer,N., Garriga,D., Martin,C.S., Velazquez-Muriel,J.A., Trus,B.L., Carrascosa,J.L., and Caston,J.R. (2007). Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch. J. Virol., 81, 6869-6878.
Agirrezabala,X., Velazquez-Muriel,J.A., Gomez-Puertas,P., Scheres,S.H.W., Carazo,J.M., and Carrascosa,J.L. (2007). Quasi-atomic model of bacteriophage T7 procapsid shell: Insights into the structure and evolution of a basic fold. Structure, 15, 461-472.
Jaime Martín-Benito, Julie Grantham, Jasminka Boskovic, Karen I. Brackley, José L. Carrascosa, Keith R. Willison y José M. Valpuesta. (2007). The inter-ring arrangement of the cytosolic chaperonin CCT. EMBO Reports. 8 (3), 252-257.
Ivanovska IL , Agirrezabala X, Carrascosa JL, Schmidt CF, Wuite GJL. (2007). Deconstructing viral capsids reveals their strengths and waknesses. Biophysical Journal : 49A Suppl.
2006
Georgia Kapatai, Andrew Large, Justin. L. P. Benesch, Carol V. Robinson, José L. Carrascosa, Jose M. Valpuesta, Preethy Gowrinathan and Peter A. Lund. (2006). All three chaperonin genes in the archaeon Haloferax volcanii are individually dispensable.
Molecular Microbiology 61 (6). 1583-1597.
José R. Castón, Daniel Luque, Benes L. Trus, Germán Rivas , Carlos Alfonso, José M. González, José L. Carrascosa, Padmanaban Annamalai, Said A. Ghabrial. (2006). Tree-dimensional structure and stoichiometry of Helmintosporium victoriae190S totivirus. Virology 347. 323-332.
2005
Agirrezabala,X., Martin-Benito,J., Caston,J.R., Miranda,R., Valpuesta,J.M., and Carrascosa,J.L. (2005). Maturation of phage T7 involves structural modification of both shell and inner core components. EMBO J., 24, 3820-3829.
Agirrezabala,X., Martin-Benito,J., Valle,M., Gonzalez,J.M., Valencia ,A., Valpuesta,J.M., and Carrascosa,J.L. (2005). Structure of the connector of bacteriophage T7 at 8A resolution: structural homologies of a basic component of a DNA translocating machinery. J Mol Biol, 347, 895-902.
Cyrklaff,M., Risco,C., Fernandez,J.J., Jimenez,M.V., Esteban,M., Baumeister,W., and Carrascosa,J.L. (2005). Cryo-electron tomography of vaccinia virus. Proc. Natl. Acad. Sci. U. S. A, 102, 2772-2777.
Saugar, I. , Luque,D., Ona,A., Rodriguez,J.F., Carrascosa,J.L., Trus,B.L., and Caston,J.R. (2005). Structural polymorphism of the major capsid protein of a double-stranded RNA virus: an amphipathic alpha helix as a molecular switch. Structure., 13, 1007-1017.
Bertrand,S.; Barthelemy, I. ; Oliva,M.A.; Carrascosa,J.L.; Andreu,J.M.; Valpuesta,J.M. (2005). Folding, stability and polymerization properties of FtsZ chimeras with inserted tubulin loops involved in the interaction with the cytosolic chaperonin CCT and in microtubule formation . J. Mol. Biol . Vol. 346 . pp. 319-330.
J. Querol-Audi, R. Pérez-Luque, I. Fita, C. López-Iglesias, J.R. Castón, J.L. Carrascosa and N. Verdaguer (2005). Preliminary analysis of two and three-dimensional crystals of vault ribonucleoprotein particles. J. Struct. Biol . Vol. 151 , pp. 111-115.
2004
I. L. Ivanovska, P. J. de Pablo, B. Ibarra, G. Sgalari, F. C. MacKintosh, J. L. Carrascosa, C. F. Schmidt, and G. J. L. Wuite (2004). Bacteriophage capsids: Tough nanoshells with complex elastic properties. Proc. Natl. Acad. Sci. USA . 101 , 7600-7605..
Barcena,J.; Verdaguer,N.; Roca,R.; Morales,M.; Angulo,I.; Risco,C.; Carrascosa,J.L.; Torres,J.M.; Caston,J.R. (2004). The coat protein of Rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid. Virology 322 , 118-134.
J. Martín-Benito, S. Bertrand, T. Hu, P. J. Ludtke, J. N. McLaughlin, B. M. Willardson, J. L. Carrascosa and J. M. Valpuesta. (2004). Structure of the complex between the cytosolic chaperonin CCT and phosducin-like protein. Proc. Natl. Acad. Sci. USA , 101, 17410-17415.
P. Gómez-Puertas, J. Martín-Benito, J. L. Carrascosa, K. R. Willison and J. M. Valpuesta (2004). The substrate recognition mechanisms in chaperonins. J. Mol. Recognit . 17 . 85- 94.
Area, E.; Martin-Benito, J.; Gastaminza, P.; Torreira, E.; Valpuesta, J.M.; Carrascosa, J.L.; Ortin, J. (2004). 3D structure of the influenza virus polymerase complex: localization of subunit domains. Proc. Natl. Acad. Sci. USA . 101 . 308- 313.
2003
IJ Salanueva, RR Novoa, P Cabezas, C LopezIglesias, JL Carrascosa, RM Elliott, C Risco (2003). Polymorphi sm and structural maturation of Bunyamwera virus in golgi and post-golgi compartments. Journal of Virology 77 , Iss 2, pp 1368-1381.
J.R. Castón, S.A. Gabrial, D. Jiang, G. Rivas, C. Alfonso, R. Roca, D. Luque and J.L. Carrascosa (2003). Three-dimensional structure of Penicillium chrysogenum virus: A double-strnded RNA virus with a genuine T=1 capsid. J. Mol. Biol . 331 , 417-431.
J.C. Gallego-Gómez, C. Risco, D. Rodríguez, P- Cabezas, S. Guerra, J.L. Carrascosa and M. Esteban. (2003). Differences in virus-induced cell morphology and in virus maturation between MVA and other strains (WR, Ankara and NYCBH) of vaccinia virus in infected human cells. J. Virol . 77 , 10606-10622.
C. Risco, J.L. Carrascosa and T. Frey. (2003). Structural maturation of rubella virus in the Golgi complex. Virology 312 , pp 261-269.
Area,E.; Martin-Benito,J.; Gastaminza,P.; Torreira,E.; Valpuesta,J.M.; Carrascosa,J.L.; Ortin,J. (2004) .3D structure of the influenza virus polymerase complex: localization of subunit domains. Proc. Natl. Acad. Sci. USA . 101 , 308-313.
P. Gómez-Puertas, J. Martín-Benito, J. L. Carrascosa, K. R. Willison and J. M. Valpuesta (2004). The substrate recognition mechanisms in chaperonins. J. Mol. Recognit . 17 , 85-94.
Active Funding
2012-2014
Analisis estructural integrado a distintos niveles de resolución: Estudio de sistemas virales modelo.
BFU2011-29038-C02-01.
I.P.: J.L. Carrascosa.
2011-2015
Transnational access and enhancement of integrated Biological Structure determination at Synchrotron X-ray radiation facilities.
FP7-Infrastructures-2011-1: 283570 BioStruct-X
Participant: J.L. Carrascosa.
2011-2015.
Multifuncional nanotechnology for selective detection and treatment of cáncer.
FP7-NMP-2010-Large-4: 262943-2 MULTIFUN
C-NMP/0878 European Union. CSIC.
José L. Carrascosa.
2010-2014.
Nuevos materiales y dispositivos biofuncionales híbridos en Nanociencia. NOBIMAT-M.
S2009MAT-1507. Programa I+D de la Comunidad de Madrid en Tecnologías 2009. CSIC.
José L. Carrascosa (9).
2009-2011.
Soporte al Comité Nacional de la IUPAB.
ACI2008-0805. Programa Nacional de Internacionalización I + D. MCIN.
José L. Carrascosa (6).
2008-2012.
Consolider-Ingenio 2010: Nanociencia Molecular.
CSD2007-00010
José L. Carrascosa (10).
2009-2011.
Análisis de las bases moleculares del proceso de ensamblaje y maduración de partículas virales.
BFU2008-02328/BMC
José L. Carrascosa (10).
2006-2009
Synthetic Biomimetic Nanoengines: A modular Platform for Engineering of Nanomechamical Actuator Building blocks. (NANOMOT).
UE; 6 FP; NEST-029084. CSIC
José L. Carrascosa (6).
2006-2009
Análisis de estructura y función de nanomáquinas y otros sistemas biológicos y su integración mediante técnicas de manipulación de moléculas individuales.
CAM. S-0505/MAT/0283-05. CSIC
José L. Carrascosa (10).
2006-2009
A multidisciplinary approoach to determine the structures of protein complexes in a model organism. 3D-Repertoire.
BFU2006-26086E. Programa Nacional de Biología Fundamental. MEC. CSIC
José L. Carrascosa.
2005-2009.
A multidisciplinary approoach to determine the structures of protein complexes in a model organism.
3D-Repertoire.
LSHG-CT-2005-512028. European Union. Framework Program 6. CSIC
José L. Carrascosa (18).
2004-2009
New electron microscopy approaches for studying protein complexes and cellular supramolecular architecture.Acronym: 3D-EM.
LifeSci/NoE/FP6-502828. European Union, Framework Program 6. CSIC
José L. Carrascosa (15).
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