Generation of transgenic mice with Yeast Artifial Chromosomes
(YACs) and Bacterial Artificial Chromosomes (BACs)
Alternative method for producing YAC-DNA transgenic mice:
Efficient generation of transgenic mice with intact
yeast artificial chromosomes by intracytoplasmic sperm injection.
Moreira PN, Giraldo P, Cozar P, Pozueta J, Jiménez A, Montoliu L, Gutiérrez-Adán
A.
Biol Reprod. 2004
Dec;71(6):1943-7. Epub 2004 Jul 30.
PDF
Visit the Transgenic Animal Model Core of the University of Michigan for an additional protocol of BAC DNA preparation for microinjection
Methods for preparing plasmid DNA for mouse oocyte
microinjection
Preparing genomic DNA and analysing by PCR
Chi-Square Tests (observed/expected distribution of
genotypes/phenotypes in mouse crosses involving 1, 2 or 3 unlinked loci)
Reference:
Mendel: a simple excel workbook to compare the
observed and expected distributions of genotypes/phenotypes in transgenic and
knockout mouse crosses involving up to three unlinked loci by means of a χ(2)
test.
Montoliu L.
Transgenic Res. 2012 Jun;21(3):677-81.
Epub 2011 Aug 19.
PDF
Cryopreservation of sperm and mouse embryos (WEB page in "Transgenesis in Mammals")