Transgenesis y Edición Genética

The Transgenesis and Genetic Edition Service is a joint scientific service of CNB and CBMSO that provides support to internal and external research groups in the creation, interchange, and management of genetically modified mouse models.

The service covers all the required steps for this purpose, from founder generation to breeding and management of lines. Models are generated by additive transgenesis, ES cell-derived gene targeting, and CRISPR/Cas9-based genome edition, either with embryo microinjection for large insertions or electroporation for KO or small insertion models.

We offer technical and scientific support, complementing the expertise of our customers, advising on the best strategy to obtain the desired model, and also providing genotyping support if needed.

The service is integrated into the scientific-technological platform INNOTEK (UAM+CSIC). We also provide rederivation of mouse lines and on-demand design and testing of guides for the genome edition of embryos and mammalian cell lines. The general activity of the service is complemented by the organization and participation in specialization courses and master programs related to genetically altered animals.

Personnel