Cristalografía de Rayos X

Protein X-ray crystallography is a high-resolution technique that enables the study of protein structure at the atomic level. It offers a comprehensive insight into protein functionality, ligand-protein interactions, supramolecular organization, and mutants linked to human disease. Significant advancements have been made in both crystallization techniques and structure resolution and refinement software over the past decade, thereby enhancing the probability of determining the structure of a macromolecule.

The Macromolecular Crystallography Facility offers the following techniques:

– Consultancy and supervision of protein production from cloning to expression in bacterial, yeast, and eukaryotic systems.

– Assistance and training in protein purification to obtain crystalline-quality protein for crystallization.

– Automated macromolecular crystallization.

– Optimization of crystallization conditions using standard and in-house techniques.

– Crystal mounting. Access to synchrotron beam time. X-ray diffraction data acquisition.