The facility hosts a cryoelectron microscope, a 200 kV FEI TALOS Arctica, equipped with an autoloader and a Falcon 4i direct electron detector. The microscope is suited for the collection of large amounts of high-resolution data that can be used for high-resolution studies using single-particle methodology, or for the reconstruction of pleiotropic structures such as cells and subcellular structures using cryoelectron tomography (cryoET). The Talos Arctica is also equipped with a Ceta-D camera for acquisition of electron diffraction data (microED). Additionally, the facility hosts a standard 120 kV electron microscope JEOL JEM1400 for sample screening.

Specimen vitrification is also available at the service, with three devices suited for vitrification of small specimens such as proteins and macromolecular complexes (a FEI Vitrobot Mark IV, a Leica EM CPC and a LEICA EM GP2), and a forth device suited for the fast-freezing of cellular samples, a Leica ICE high-pressure freezer.

This facility is unique in Spain as it also offers the newly developed cryocorrelative microscopy technique (cryoCLEM), which allows the analysis of the same biological specimen by cryooptical microscopy (Zeiss LS900 AiryScan cryoconfocal microscope) and cryoelectron microscopy (both cryoelectron microscopes described above). The use of a Zeiss CrossBeam 550 cryo-FIB-SEM microscope will increase the cryocorrelative microscopy capabilities of the facility to direct visualization of cells for tissue-cell resolution or for preparation of thin lamellas in cells for molecular resolution.