Histología
SERVICIOS
Lluís Montoliu
Head of Histology
The CNB histology facility offers the preparation of animal and plant biological samples for their histological analyses.
All requests are received and processed electronically, through the established facility’s registration procedure at the devoted web site, available in Spanish and in English. Offered methods and procedures include the preparation of wax (paraffin) and plastic (resin) blocks with biological specimens embedded, and the corresponding generation of histological sections with one of the two available automated microtomes.
The CNB histology facility has an ample experience in processing a large variety of animal and plant tissues and organs. The CNB histology facility coordinates a joint platform with the IIB-UAM/CSIC histology facility, offering to CNB and IIB researchers a larger processing capacity for histological samples.
Equipment
1 motorized Microtome (plastic/resin/paraffin/wax) Leica RM2155
1 motorized Microtome (plastic/resin/paraffin/wax) Thermo Scientific, Shandon, FINESSE ME+
1 Tissue Processing Station Leica PEARL
1 Cryostat Leica CM1950
1 Tissue Embedding Station Leica EG1160
2 thermostatic baths Leica HI1210
1 stereo microscope Leica M80
1 Optical Microscope Leica DM4-B
1 drying oven Raypa
Techniques
The Histology facility offers the preparation and sectioning of frozen blocks with the cryostat. The orientation, width and arrangement of the sections can be specified by the user. All sections can be counterstained with any of the available staining procedures (haematoxylin/eosin, cresyl violet, PAS, Mason’s trichrome, elastin fibres/Van Gieson/Sirius Red, etc.) or can be processed subsequently for immunohistochemistry. The facility implements new staining procedures or histological methods upon request.
Personnel
Technical manager
Soledad Montalbán
Óscar Sánchez
