Citometría de Flujo
SERVICIOS
Miguel Ángel Sánchez Luengo
Head of Flow Citometry
Flow Cytometry is a technique of cell analysis multiparametric whose purpose is the detection and identification of molecules and cellular structures thanks to the use of fluorescent markers and conjugated antibodies. Flow sorting is an extension of this technology, by which any cell or object measured can be separated selectively from the suspension based on properties measured in flow.
The Facility provides scientific and technological support to the different CNB research groups that require it as well as to researchers from the public and private sectors The Unit offers: training and advice on the principles and the applications of the analytical flow cytometry. To develop and optimize new applications, incorporating new technologies and new reagents. Quantification of secreted cytokines by multiplexed assays. Result’s analysis using specialized software packages. Cellular isolation by Cell Sorting. Antibodies and commonly used reagents
Equipment and Services
1 Digital analyzer Beckman Coulter CYTOMICS FC 500: 5 fluorescence detectors, 1 laser (488nm).
1 Digital analyzer BD LSRII: 8 fluorescence detectors, 3 lasers (488nm, 633nm and 405nm).
1 Digital analyzer Beckman Coulter GALLIOS Analyzer: 10 fluorescence detectors, 3 lasers (488nm, 633nm and 405nm).
1 Digital analyzer Beckman Cytoflex S: 13 fluorescence detectors, 4 lasers (488nm, 561nm, 633nm and 405nm)
1 Digital analyzer Beckman Cytoflex :13 fluorescence detectors, 3 lasers (488nm, 633nm and 405nm)
1 Spectral analyzer Cytek Aurora: up to 40 colors detection, 5 lasers (355nm, 404nm, 488nm, 561nm and 640nm)
1 Multiplex protein quantitation system LUMINEX 100 IS.
1 Cell Sorter Beckman Coulter Moflow XDP: 10 fluorescence detectors, 3 lasers (488nm, 633nm and 405nm).
1Cell Sorter Beckman Cytoflex SRT: 15 fluorescence detectors, 4 lasers (488nm, 561nm, 633nm and 405nm)
Personnel
Head
Miguel Ángel Sánchez Luengo
Technician
Teresa Osteso Ibáñez
