Criopreservación de Embriones de Ratón

The CNB mouse embryo cryopreservation facility offers to researchers the possibility to freeze, maintain and rescue transgenic and knockout mouse lines in the form of embryos and/or sperm, hence contributing to current animal welfare recommendations and complying with the associated legislation on animal experimentation. Current methods available include freezing sperm, oocytes and/or embryos, the thawing of sperm, oocytes and/or embryos previously frozen and the subsequent revitalisation of the cryopreserved mouse lines through in vitro fertilisation, assessment and/or logistical support for importing/exporting frozen or refrigerated embryos or sperm, from and to the CNB, and quality controls and genotyping procedures. The facility can also produce genome-edited mouse models using the latest CRISPR-Cas9 tools through embryo electroporation.

The CNB hosts the Spanish node of the European scientific research infrastructure (ESFRI) called INFRAFRONTIER, which includes the European Mouse Mutant Archive (EMMA), and whose objective is the generation, phenotyping, cryopreservation, organised archiving and coordinated distribution of mouse lines of interest in biomedicine. EMMA has more than 8,400 mouse mutant lines cryopreserved and is composed of 16 nodes that are present in 13 European countries. More than 550 of those mouse lines are cryopreserved and offered from the Spanish node at CNB. The CNB mouse embryo cryopreservation facility has signed scientific cooperation agreements with the Spanish National Cancer Centre (CNIO) and with the Centre for Animal Resources and Development (CARD) at the University of Kumamoto (Japan) for the archiving and distribution of mutant mouse lines of interest in biomedical research.

Personnel