Transgénesis

BelenPindado

 

Mª Belén Pintado Sanjuanbenito

Group Leader

Contact

 

Contacto:

 


 

Descripción del servicio

Trangenesis

El servicio de transgénesis es una unidad compartida entre el CNB y CBMSO e integrada en el Campus Internacional de Excelencia UAM+CSIC. Su misión es proporcionar apoyo a los grupos de investigación en la creación, establecimiento o intercambio de estirpes de raton genéticamente alteradas. La unidad ofrece asesoramiento y apoyo científico y técnico para la obtención del modelo de ratón mas adecuado, bien por transgénesis aditiva, mutagénesis dirigida (modelos KO y KI), o edición genómica (CRISPr, TALENs o ZFNs). La unidad también realiza rederivaciones sanitarias para incorporar lineas procedentes de intercambios cuando hay problemas de estado sanitario en el animalario original, genotipaciones bajo demanda  y obtención de embriones y líneas ES.

 

 

Transgenesis   Logo A CEI blanco   66 CSIC cmyk LogoPeqCBMSO

 

 

 

grupoTransgenesisRed 

Name
Position
Contact
Mª Belén Pintado Sanjuanbenito Lead investigator
Marta García Flores Personnel
Verónica Domínguez Plaza Personnel CBMSO
Alicia Llorente Personnel
Laura López Martínez Personnel CBMSO

 

 

  • Rederivación de embriones de ratón
  • Generación de ratones transgénicos (Transgénesis aditiva)
  • Generación de modelos de ratón KO y KI
    • Electroporación de células ES
    • Microinyección de células ES propias o procedentes de consorcios internacionales.
    • Microinyección de CRISPRs /Cas9 en embriones de ratón.
  • Establecimiento de líneas genéticamente modificadas
    • Gestión de quimeras y fundadores
    • Genotipado por PCR y Long PCR
    • Test de homocigosis mediante real-time PCR
  • Generación de líneas de células ES
  • Test de Mycoplasma en cultivos celulares

ratones    embrion

 

PRESUPUESTOS ORIENTATIVOS (en Euros)
     CNB-CBMSO      CSIC      OPI/UNIVERSIDAD      OTROS (Privado)  

 

REDERIVACIÓN (SESIÓN):    

Incluye: recogida de embriones y transferencia a hembras donantes. 240 300 500 600

 

MICROINYECCIÓN DE DNA ó CRISPR EN PRONUCLEO O CITOPLASMA DE EMBRIONES (Proyecto)    

Incluye: recogida de embriones, microinyección y transferencia a hembras donantes. 1650 2000 3100 3360

 

MICROINYECCIÓN DE CÉLULAS ES DE CONSORCIOS EN EMBRIONES (tres sesiones)    

Incluye cultivo de ES y control sanitario, recogida de embriones, microinyección y transferencia a hembras donantes. 1951 2215 2373 2559

 

ELECTROPORACIÓN DE VECTORES DE MUTAGÉNESIS EN CÉLULAS ES    

General 1171 1374 1519 1663
En Rosa-26 394 462 511 560

 

MICROINYECCIÓN DE CÉLULAS ES DEL SERVICIO EN EMBRIONES (2 sesiones)    

Inyección en embriones de 8 células 617 724 1167 1278
Inyección de blastos 1275 1496 2020 2213

 

GESTIÓN DE COLONIAS DE RATONES MODIFICADOS GENETICAMENTE EN NUESTRO SERVICIO (Por fundador/Quimera)    

Cruce de las quimeras/fundadores para establecimiento de línea en heterocigosis 210.37 246.77 342.23 374.82
Genotipación de la descendencia por PCR estándard (25 muestras) 65.75 77 130.75 143.25

 

GENOTIPACIÓN    

PCR standard (por muestra) 2.63 3.08 5.23 5.73
Long range PCR (por muestra) 3.41 4 6.25 6.84

 

Para Test de homocigosis y test de Mycoplasma, consultar directamente con el servicio.

TABLA DETALLADA DE PRECIOS OFICIALES: intranet CSIC/catálogo de prestaciones.

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