Mouse embryo cryopreservation facility

LMontoliu

 

Lluís Montoliu

Lead Scientist

Contact

 

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Service Description

Pajuelas nitrogeno criopreservacionRed

The CNB Mouse Embryo Cryopreservation Facility offers researchers the possibility of freezing and maintaining transgenic and knockout mouse lines in the form of embryos and/or sperm. Additional services available include the thawing of sperm and/or embryos and revitalization of cryopreserved mouse lines, in vitro fertilization, assessment and/or logistical support for importing/exporting frozen or refrigerated embryos or sperm to and from the CNB, quality controls and genotyping procedures. Cryopreservation of mouse lines is a highly recommended process for archiving animal models used in biology, biomedicine and biotechnology for long periods of time, The conditions are stable and safe, and dispense with the need to maintain mouse lines alive and breeding, therefore saving space, funds and reducing the number of animals in experiments, according to current animal welfare regulations. The National Centre for Biotechnology (CNB-CSIC) hosts the Spanish node of the European Project INFRAFRONTIER-EMMA, whose objective is the cryopreservation, organised archiving and coordinated distribution of mouse lines of interest for the scientific community in Biomedicine.

 

The CNB-CSIC and CNIO signed a scientific cooperation agreement to allow archiving and distribution of mutant mouse lines of interest in biomedical research, generated by CNIO researchers, through the INFRAFRONTIER-EMMA project. The CNB Mouse Embryo Cryopreservation Facility is integrated in the scientific-technological platforms INNOTEK, from the Campus of Excellence UAM+SIC, and SEFALER of the CIBERER/ISCIII.

The CSIC and the IRDA-University of Kumamoto signed an academic cooperation agreement to promote the exchange of knowledge, personnel and information on the mouse embryos and sperm archiving and cryopreservation activities undertaken at the Spanish node of INFRAFRONTIER-EMMA at the CNB-CSIC and at the CARD archive, coordinated by Dr Lluís Montoliu and Prof Naomi Nakagata, respectively. The first initiative derived from this cooperation took place in October 2013 with the International CARD-CNB Mouse Embryo and Sperm Cryopreservation Course, organized at the CNB by Naomi Nakagata and Lluís Montoliu.

 

Publication

INFRAFRONTIER-providing mutant mouse resources as research tools for the international scientific community. INFRAFRONTIER Consortium. Nucleic Acids Res 2015; 43(Database issue):D1171-5

 

Fees and others

 

Criopreservacion Feb2015Red 

Name
Position
Contact
Lluís Montoliu Lead scientist
Juliana Fernández Punzano Personnel
María Jesús del Hierro Sánchez Personnel
Marta Castrillo Labrador Personnel
Isabel Martín-Dorado Caballero Personnel

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