• Anfibios
• Peces

• Pre-Dolly
• Dolly
• Ovejas
• Vacas
• Cabras
• Cerdos
• Conejos
• Gatos
• Perros
• Ratones
• Ratas
• Equinos
• Hurones
• Revisiones
• Problemas y riesgos
• Gemelación artificial

            (Textos legales I a VII, obtenidos a partir de la WEB del Prof. J.R.Lacadena)

• I. LEY 35/1988, DE 22 DE NOVIEMBRE, SOBRE TÉCNICAS DE REPRODUCCIÓN ASISTIDA (Archivo PDF) (Modificada por Ley 45/2003, de 21 de noviembre) (Derogada por Ley 14/2006, de 26 de mayo)
• II. LEY 42/1988, DE 28 DE DICIEMBRE, SOBRE DONACIÓN Y UTILIZACIÓN DE EMBRIONES Y FETOS HUMANOS O DE SUS CÉLULAS, TEJIDOS U ÓRGANOS. (Archivo PDF)
• III. LEY ORGÁNICA 10/1995, DE 23 DE NOVIEMBRE, DEL CÓDIGO PENAL. (Archivo PDF)
• IV. REAL DECRETO 412/1996, de 1 de marzo, por el que se establecen los protocolos obligatorios de estudio de los donantes y usuarios relacionados con lastécnicas de reproducción humana asistida y se regula la creación y organización del Registro Nacional de Donantes de Gametos y Preembriones con fines de reproducción humana. (Archivo PDF)
• V. REAL DECRETO 413/1996, de 1 de marzo, por el que se establecen los requisitos técnicos y funcionales precisos para la autorización y homologación de los centros y servicios sanitarios relacionados con las técnicas de reproducción humana asistida. ORDEN de 25 de marzo de 1996 por la que se establecen las Normas de funcionamiento del Registro Nacional de Donantes de Gametos y Preembriones. (Archivo PDF)
• VI. ORDEN de 25 de marzo de 1996 por la que se establecen las Normas de funcionamiento del Registro Nacional de Donantes de Gametos y Preembriones.(BOE 2-5-1996, núm. 106, pág. 15469) (Archivo PDF)
• VII. REAL DECRETO 415/1997, de 21 de marzo, por el que se crea la Comisión Nacional de Reproducción Humana Asistida. (Archivo PDF)

 

LEY 45/2003, de 21 de NOVIEMBRE, por la que se modifica la Ley 35/1988, de 22 de noviembre, sobre TECNICAS DE REPRODUCCION ASISTIDA

(Derogada por Ley 14/2006, de 26 de mayo)

REAL DECRETO 1720/2004, de 23 de julio, por el que se establecen las tipologías fisiopatólogicas que permiten la superación de los límites generales establecidos para la fecundación de ovocitos en procesos de reproducción asistida.

EL CONSEJO DE MINISTROS APRUEBA LA LEY DE REPRODUCCIóN ASISTIDA (6 de mayo de 2005)

Ley sobre técnicas de reproducción asistida humana (Ley 14/2006, de 26 de mayo, BOE n. 126, sábado 27 de mayo de 2006, pag. 19947-19956)

Ley de Investigación Biomédica (Ley 14/2007, de 3 de julio, BOE n. 159, miércoles 4 julio de 2007, pag. 28826-28848)

Comunidades Autónomas:

JUNTA DE ANDALUCÍA:

1) Ley 7/2003, de 20 de octubre, por la que se regula investigación en Andalucía con preembriones humanos no viables para la fecundación in vitro (B.O.J.A. de 31 de octubre de 2003, num. 210, pag. 22734 y siguientes). (Archivo PDF) (Publicación de la Ley 7/2003, de 20 de octubre, en el B.O.E. nº 279, 21 de noviembre de 2003, pag. 41238-41241)-

Legislación sobre ANIMALES DE EXPERIMENTACIÓN y ORGANISMOS MODIFICADOS GENÉTICAMENTE(Europea / Nacional)

ESPAÑA: Comisión Nacional de Reproducción Humana Asistida (Ministerio de Sanidad y Consumo)

• Resumen (I Informe Anual, Diciembre 1998)
• I Informe completo, I Informe Anual, Diciembre 1998, (7.3 Mb, PDF)
• II Informe (WEB del Prof. JR Lacadena)
• Resumen del II Informe de la CNRHA (archivo PDF)

• Informe sobre células troncales del Comité Asesor de Ética en la Investigación Científica y Tecnológica (archivo PDF)
• Comite Asesor de Ética en la Investigación Científica y Tecnológica

Fundación Europea para la Ciencia  (European Science Foundation)

• Human Stem Cells Research (news release)
• Human Stem Cells Research (full report, PDF file)

Legislación sobre embriones/células troncales en otros paises europeos (BIONET)
"A survey on the National Regulations in the Europe Union regarding Research on Human Embryos" (Brigitte Gratton, EGE) (Archivo PDF)

Evers K.
European perspectives on therapeutic cloning.
N Engl J Med. 2002 May 16;346(20):1579-82. (Archivo PDF)

REPORT ON EMBRYONIC STEM CELL RESEARCH BY UNESCO’S INTERNATIONAL BIOETHICS COMMITTEE (Feb 2001)

International Bioethics Committee (UNESCO)

Universal Declaration on the Human Genome and Human Rights

Número especial de la revista SCIENCE
25 de febrero de 2000
N. 5457
Vol. 287
Stem cells: research and ethics

Amphibians / Anfibios

Spemann H (1938)
Embryonic development and induction (Yale Univ. Press, New Haven, CT)

Briggs R, King TJ (1952)
Transplantation of living nuclei from blastula cells intro enucleated frogs' egss
Proc. Natl. Acad. Sci. USA 38: 455-463.

Briggs R, King TJ (1957)
Changes in the nuclei of differentiating endoderm cells as revealed by nuclear transplantation
J. Morphol. 100: 269-311.

McKinnell RG (1962)
Intraspecific nuclear transplantation in frogs
J. Hered. 53: 199-207.

Gurdon JB, Uehlinger V.
"Fertile" intestine nuclei.
Nature. 1966 Jun 18;210(42):1240-1.

Hoffner NJ, DiBerardino MA.
Developmental potential of somatic nuclei transplanted into meiotic oocytes of Rana pipiens.
Science. 1980 Jul 25;209(4455):517-9.

DiBerardino MA, Hoffner NJ.
Gene reactivation in erythrocytes: nuclear transplantation in oocytes and eggs of Rana.
Science. 1983 Feb 18;219(4586):862-4.

DiBerardino MA, Orr NH, McKinnell RG.
Feeding tadpoles cloned from Rana erythrocyte nuclei.
Proc Natl Acad Sci U S A. 1986 Nov;83(21):8231-4.

Gurdon JB.
Nuclear transplantation in eggs and oocytes.
J Cell Sci Suppl. 1986;4:287-318.

Fish / Peces

Lee KY, Huang H, Ju B, Yang Z, Lin S.
Cloned zebrafish by nuclear transfer from long-term-cultured cells.
Nat Biotechnol. 2002 Aug;20(8):795-9.

Murphey RD, Zon LI.
Attack of the fish clones.
Nat Biotechnol. 2002 Aug;20(8):785-6.
 

CLONING IN MAMMALS (clonación en mamíferos) 
 

Pre-Dolly

McGrath J, Solter D.
Nuclear transplantation in the mouse embryo by microsurgery and cell fusion.
Science. 1983 Jun 17;220(4603):1300-2.

Willadsen SM.
Nuclear transplantation in sheep embryos.
Nature. 1986 Mar 6-12;320(6057):63-5.

Sims M, First NL.
Production of calves by transfer of nuclei from cultured inner cell mass cells.
Proc Natl Acad Sci U S A. 1993 90:6143-7.

Kwon OY, Kono T.
Production of identical sextuplet mice by transferring metaphase nuclei from four-cell embryos.
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13010-3.

Campbell KH, McWhir J, Ritchie WA, Wilmut I.
Sheep cloned by nuclear transfer from a cultured cell line.
Nature. 1996 Mar 7;380(6569):64-6.
 

Dolly

Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH.
Viable offspring derived from fetal and adult mammalian cells.
Nature. 1997 Feb 27;385(6619):810-3.

Solter D.
Dolly is a clone--and no longer alone.
Nature. 1998 Jul 23;394(6691):315-6.

Signer EN, Dubrova YE, Jeffreys AJ, Wilde C, Finch LM, Wells M, Peaker M.
DNA fingerprinting Dolly.
Nature. 1998 Jul 23;394(6691):329-30.

Ashworth D, Bishop M, Campbell K, Colman A, Kind A, Schnieke A, Blott S, Griffin H, Haley C, McWhir J, Wilmut I.
DNA microsatellite analysis of Dolly.
Nature. 1998 Jul 23;394(6691):329.

Shiels PG, Kind AJ, Campbell KH, Waddington D, Wilmut I, Colman A, Schnieke AE.
Analysis of telomere lengths in cloned sheep.
Nature. 1999 May 27;399(6734):316-7.

Evans MJ, Gurer C, Loike JD, Wilmut I, Schnieke AE, Schon EA.
Mitochondrial DNA genotypes in nuclear transfer-derived cloned sheep.
Nat Genet. 1999 Sep;23(1):90-3.
 

Scheep / Ovejas

Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, Wilmut I, Colman A, Campbell KH.
Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts.
Science. 1997 Dec 19;278(5346):2130-3.

Wells DN, Misica PM, Day AM, Peterson AJ, Tervit HR.
Cloning sheep from cultured embryonic cells.
Reprod Fertil Dev. 1998;10(7-8):615-26.

McCreath KJ, Howcroft J, Campbell KH, Colman A, Schnieke AE, Kind AJ.
Production of gene-targeted sheep by nuclear transfer from cultured somatic cells.
Nature. 2000 Jun 29;405(6790):1066-9.

Denning C, Burl S, Ainslie A, Bracken J, Dinnyes A, Fletcher J, King T, Ritchie M, Ritchie WA, Rollo M, de Sousa P, Travers A, Wilmut I, Clark AJ.
Deletion of the alpha(1,3)galactosyl transferase (GGTA1) gene and the prion protein (PrP) gene in sheep.
Nat Biotechnol. 2001 Jun;19(6):559-62.
 

Cattle - calves - bulls / Terneras - vacas- toros

Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de Leon FA, Robl JM.
Cloned transgenic calves produced from nonquiescent fetal fibroblasts.
Science. 1998 May 22;280(5367):1256-8.

Kato Y, Tani T, Sotomaru Y, Kurokawa K, Kato J, Doguchi H, Yasue H, Tsunoda Y.
Eight calves cloned from somatic cells of a single adult.
Science. 1998 Dec 11;282(5396):2095-8.

Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de Leon FA, Robl JM.
Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells.
Nat Biotechnol. 1998 Jul;16(7):642-6.

Wells DN, Misica PM, Tervit HR.
Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells.
Biol Reprod. 1999 Apr;60(4):996-1005.

Robl JM.
Development and application of technology for large scale cloning of cattle.
Theriogenology. 1999 Jan 15;51(2):499-508.

Kubota C, Yamakuchi H, Todoroki J, Mizoshita K, Tabara N, Barber M, Yang X.
Six cloned calves produced from adult fibroblast cells after long-term culture.
Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):990-5.

Steinborn R, Schinogl P, Zakhartchenko V, Achmann R, Schernthaner W, Stojkovic M, Wolf E, Muller M, Brem G.
Mitochondrial DNA heteroplasmy in cloned cattle produced by fetal and adult cell cloning.
Nat Genet. 2000 Jul;25(3):255-7.

Zakhartchenko V, Mueller S, Alberio R, Schernthaner W, Stojkovic M, Wenigerkind H, Wanke R, Lassnig C, Mueller M, Wolf E, Brem G.
Nuclear transfer in cattle with non-transfected and transfected fetal or cloned transgenic fetal and postnatal fibroblasts.
Mol Reprod Dev. 2001 Nov;60(3):362-9.

Arat S, Rzucidlo SJ, Gibbons J, Miyoshi K, Stice SL.
Production of transgenic bovine embryos by transfer of transfected granulosa cells into enucleated oocytes.
Mol Reprod Dev. 2001 Sep;60(1):20-6.

Forsberg EJ, Strelchenko NS, Augenstein ML, Betthauser JM, Childs LA, Eilertsen KJ, Enos JM, Forsythe TM, Golueke PJ, Koppang RW, Lange G, Lesmeister TL, Mallon KS, Mell GD, Misica PM, Pace MM, Pfister-Genskow M, Voelker GR, Watt SR, Bishop MD.
Production of cloned cattle from in vitro systems.
Biol Reprod. 2002 Jul;67(1):327-33.
 

Goats / Cabras

Baguisi A, Behboodi E, Melican DT, Pollock JS, Destrempes MM, Cammuso C, Williams JL, Nims SD, Porter CA, Midura P, Palacios MJ, Ayres SL, Denniston RS, Hayes ML, Ziomek CA, Meade HM, Godke RA, Gavin WG, Overstrom EW, Echelard Y.
Production of goats by somatic cell nuclear transfer.
Nat Biotechnol. 1999 May;17(5):456-61.

Loi P, Ptak G, Barboni B, Fulka J Jr, Cappai P, Clinton M.
Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells.
Nat Biotechnol. 2001 Oct;19(10):962-4.

Keefer CL, Keyston R, Lazaris A, Bhatia B, Begin I, Bilodeau AS, Zhou FJ, Kafidi N, Wang B, Baldassarre H, Karatzas CN.
Production of cloned goats after nuclear transfer using adult somatic cells.
Biol Reprod. 2002 Jan;66(1):199-203.
 

Pigs / Cerdos

Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KH.
Cloned pigs produced by nuclear transfer from adult somatic cells.
Nature. 2000 Sep 7;407(6800):86-90.

Onishi A, Iwamoto M, Akita T, Mikawa S, Takeda K, Awata T, Hanada H, Perry AC.
Pig cloning by microinjection of fetal fibroblast nuclei.
Science. 2000 Aug 18;289(5482):1188-90.

Towards obtaining knock-out pigs (PPL-Therapeutics)
2002 Jan 2

Butler D.
Xenotransplant experts express caution over knockout piglets.
Nature. 2002 Jan 10;415(6868):103-4.

Lai L, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, Murphy CN, Carter DB, Hawley RJ, Prather RS.
Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning.
Science. 2002 Feb 8;295(5557):1089-92.

Dai Y, Vaught TD, Boone J, Chen SH, Phelps CJ, Ball S, Monahan JA, Jobst PM, McCreath KJ, Lamborn AE, Cowell-Lucero JL, Wells KD, Colman A, Polejaeva IA, Ayares DL.
Targeted disruption of the alpha1,3-galactosyltransferase gene in cloned pigs.
Nat Biotechnol. 2002 Mar;20(3):251-5.

Park KW, Lai L, Cheong HT, Cabot R, Sun QY, Wu G, Rucker EB, Durtschi D, Bonk A, Samuel M, Rieke A, Day BN, Murphy CN, Carter DB, Prather RS.
Mosaic gene expression in nuclear transfer-derived embryos and the production of cloned transgenic pigs from ear-derived fibroblasts.
Biol Reprod. 2002 Apr;66(4):1001-5.

Boquest AC, Grupen CG, Harrison SJ, McIlfatrick SM, Ashman RJ, d'Apice AJ, Nottle MB.
Production of cloned pigs from cultured fetal fibroblast cells.
Biol Reprod. 2002 May;66(5):1283-7.

Lai L, Park KW, Cheong HT, Kuhholzer B, Samuel M, Bonk A, Im GS, Rieke A, Day BN, Murphy CN, Carter DB, Prather RS.
Transgenic pig expressing the enhanced green fluorescent protein produced by nuclear transfer using colchicine-treated fibroblasts as donor cells.
Mol Reprod Dev. 2002 Jul;62(3):300-6.

Phelps CJ, Koike C, Vaught TD, Boone J, Wells KD, Chen SH, Ball S, Specht SM, Polejaeva IA, Monahan JA, Jobst PM, Sharma SB, Lamborn AE, Garst AS, Moore M, Demetris AJ, Rudert WA, Bottino R, Bertera S, Trucco M, Starzl TE, Dai Y, Ayares DL.
Production of alpha 1,3-galactosyltransferase-deficient pigs.
Science. 2003 Jan 17;299(5605):411-4.
 

Rabbits / Conejos

Li GP, Chen DY, Lian L, Han ZM, Zhu ZY, Seidel GE Jr.
Rabbit cloning: improved fusion rates using cytochalasin B in the fusion buffer.
Mol Reprod Dev. 2002 Feb;61(2):187-91.

Chesne P, Adenot PG, Viglietta C, Baratte M, Boulanger L, Renard JP.
Cloned rabbits produced by nuclear transfer from adult somatic cells.
Nat Biotechnol. 2002 Apr;20(4):366-9.

Matsuda J, Takahashi S, Ohkoshi K, Kaminaka K, Kaminaka S, Nozaki C, Maeda H, Tokunaga T.
Production of transgenic chimera rabbit fetuses using somatic cell nuclear transfer.
Cloning Stem Cells. 2002;4(1):9-19.
 

Cats / Gatos

Shin T, Kraemer D, Pryor J, Liu L, Rugila J, Howe L, Buck S, Murphy K, Lyons L, Westhusin M.
Cell biology: A cat cloned by nuclear transplantation.
Nature. 2002 Feb 21;415(6874):859.
 

Dogs / Perros

Lee BC, Kim MK, Jang G, Oh HJ, Yuda F, Kim HJ, Shamim MH, Kim JJ, Kang SK, Schatten G, Hwang WS.
Dogs cloned from adult somatic cells.
Nature. 2005 Aug 4;436(7051):641. Erratum in: Nature. 2005 Aug 25;436(7054):1102.
 

Mice / Ratones

Wakayama T, Perry AC, Zuccotti M, Johnson KR, Yanagimachi R.
Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei.
Nature. 1998 Jul 23;394(6691):369-74.

Wakayama T, Yanagimachi R.
Cloning of male mice from adult tail-tip cells.
Nat Genet. 1999 Jun;22(2):127-8.

Wakayama T, Rodriguez I, Perry AC, Yanagimachi R, Mombaerts P.
Mice cloned from embryonic stem cells.
Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14984-9.

Rideout WM, Wakayama T, Wutz A, Eggan K, Jackson-Grusby L, Dausman J, Yanagimachi R, Jaenisch R.
Generation of mice from wild-type and targeted ES cells by nuclear cloning.
Nat Genet. 2000 Feb;24(2):109-10. No abstract available.

Wakayama T, Tateno H, Mombaerts P, Yanagimachi R.
Nuclear transfer into mouse zygotes.
Nat Genet. 2000 Feb;24(2):108-9.

Wakayama T, Shinkai Y, Tamashiro KL, Niida H, Blanchard DC, Blanchard RJ, Ogura A, Tanemura K, Tachibana M, Perry AC, Colgan DF, Mombaerts P, Yanagimachi R.
Cloning of mice to six generations.
Nature. 2000 Sep 21;407(6802):318-9.

Ono Y, Shimozawa N, Muguruma K, Kimoto S, Hioki K, Tachibana M, Shinkai Y, Ito M, Kono T.
Production of cloned mice from embryonic stem cells arrested at metaphase.
Reproduction. 2001 Nov;122(5):731-6.

Yamazaki Y, Makino H, Hamaguchi-Hamada K, Hamada S, Sugino H, Kawase E, Miyata T, Ogawa M, Yanagimachi R, Yagi T.
Assessment of the developmental totipotency of neural cells in the cerebral cortex of mouse embryo by nuclear transfer.
Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):14022-6.

Inoue K, Kohda T, Lee J, Ogonuki N, Mochida K, Noguchi Y, Tanemura K, Kaneko-Ishino T, Ishino F, Ogura A.
Faithful expression of imprinted genes in cloned mice.
Science. 2002 Jan 11;295(5553):297.

Hochedlinger K, Jaenisch R.
Monoclonal mice generated by nuclear transfer from mature B and T donor cells.
Nature. 2002 Feb 28;415(6875):1035-8.

Lee J, Inoue K, Ono R, Ogonuki N, Kohda T, Kaneko-Ishino T, Ogura A, Ishino F.
Erasing genomic imprinting memory in mouse clone embryos produced from day 11.5 primordial germ cells.
Development. 2002 Apr 15;129(8):1807-1817.

Rats / Ratas

Qi Zhou, Jean-Paul Renard, Gaëlle Le Friec, Vincent Brochard, Nathalie Beaujean, Yacine Cherifi, Alexandre Fraichard, Jean Cozzi
Generation of Fertile Cloned Rats by Regulating Oocyte Activation
Science (Sciencexpress), Published online September 25, 2003
 

Equines / Equinos

Woods GL, White KL, Vanderwall DK, Li GP, Aston KI, Bunch TD, Meerdo LN, Pate BJ.
A mule cloned from fetal cells by nuclear transfer.
Science. 2003 Aug 22;301(5636):1063. Epub 2003 May 29.

Holden C.
Genetics. First cloned mule races to finish line.
Science. 2003 May 30;300(5624):1354.

Galli C, Lagutina I, Crotti G, Colleoni S, Turini P, Ponderato N, Duchi R, Lazzari G.
Pregnancy: a cloned horse born to its dam twin.
Nature. 2003 Aug 7;424(6949):635.
 

Ferrets / Hurones

Li Z, Sun X, Chen J, Liu X, Wisely SM, Zhou Q, Renard JP, Leno GH, Engelhardt JF.

Cloned ferrets produced by somatic cell nuclear transfer.

Dev Biol. 2006 May 15;293(2):439-48.

Reviews / Revisiones

Wilmut I.
Cloning for medicine.
Sci Am. 1998 Dec;279(6):58-63.

Wilmut I, Young L, Campbell KH.
Embryonic and somatic cell cloning.
Reprod Fertil Dev. 1998;10(7-8):639-43.

Wilmut I, Campbell KH.
Quiescence in nuclear transfer.
Science. 1998 Sep 11;281(5383):1611.

Gurdon JB, Colman A.
The future of cloning.
Nature. 1999 Dec 16;402(6763):743-6.

Colman A, Kind A.
Therapeutic cloning: concepts and practicalities.
Trends Biotechnol. 2000 May;18(5):192-6.

Solter D.
Mammalian cloning: advances and limitations.
Nat Rev Genet. 2000 Dec;1(3):199-207.

Westhusin ME, Long CR, Shin T, Hill JR, Looney CR, Pryor JH, Piedrahita JA.
Cloning to reproduce desired genotypes.
Theriogenology. 2001 Jan 1;55(1):35-49.

Wolf DP, Mitalipov S, Norgren RB Jr.
Nuclear transfer technology in mammalian cloning.
Arch Med Res. 2001 Nov-Dec;32(6):609-13.

Dinnyes A, De Sousa P, King T, Wilmut I.
Somatic cell nuclear transfer: recent progress and challenges.
Cloning Stem Cells. 2002;4(1):81-90

Hochedlinger K, Jaenisch R.
Nuclear transplantation: lessons from frogs and mice.
Curr Opin Cell Biol. 2002 Dec;14(6):741-8.

Solter D.
Cloning v. clowning.
Genes Dev. 2002 May 15;16(10):1163-6.

Gurdon JB, Byrne JA.
The first half-century of nuclear transplantation.
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8048-52. Epub 2003 Jun 23.

Loi P, Fulka J, Ptak G.
Amphibian and mammal somatic-cell cloning: different species, common results?
Trends Biotechnol. 2003 Nov;21(11):471-3.

Jaenisch R, Hochedlinger K, Blelloch R, Yamada Y, Baldwin K, Eggan K.
Nuclear cloning, epigenetic reprogramming, and cellular differentiation.
Cold Spring Harb Symp Quant Biol. 2004;69:19-27.
 

Wilmut I.

An astonishing experiment.

Cloning Stem Cells. 2006 Winter;8(4):235-6.

Troubles and risks with cloning in mammals / Problemas y riesgos de clonación en mamíferos
 

Young LE, Sinclair KD, Wilmut I.
Large offspring syndrome in cattle and sheep.
Rev Reprod. 1998 Sep;3(3):155-63.

Hill JR, Roussel AJ, Cibelli JB, Edwards JF, Hooper NL, Miller MW, Thompson JA, Looney CR, Westhusin ME, Robl JM, Stice SL.
Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies).
Theriogenology. 1999 Jun;51(8):1451-65.

Solter D.
Mammalian cloning: advances and limitations.
Nat Rev Genet. 2000 Dec;1(3):199-207.

Sinclair KD, Young LE, Wilmut I, McEvoy TG.
In-utero overgrowth in ruminants following embryo culture: lessons from mice and a warning to men.
Hum Reprod. 2000 Dec;15 Suppl 5:68-86.

Kikyo N, Wolffe AP.
Reprogramming nuclei: insights from cloning, nuclear transfer and heterokaryons.
J Cell Sci. 2000 Jan;113 ( Pt 1):11-20.

Bourc'his D, Le Bourhis D, Patin D, Niveleau A, Comizzoli P, Renard JP, Viegas-Pequignot E.
Delayed and incomplete reprogramming of chromosome methylation patterns in bovine cloned embryos.
Curr Biol. 2001 Oct 2;11(19):1542-6.

Young LE, Fernandes K, McEvoy TG, Butterwith SC, Gutierrez CG, Carolan C, Broadbent PJ, Robinson JJ, Wilmut I, Sinclair KD.
Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture.
Nat Genet. 2001 Feb;27(2):153-4.

Jaenisch R., Wilmut I
Developmental biology. Don't clone humans!
Science. 2001 Mar 30;291(5513):2552.

De Sousa PA, King T, Harkness L, Young LE, Walker SK, Wilmut I.
Evaluation of gestational deficiencies in cloned sheep fetuses and placentae.
Biol Reprod. 2001 Jul;65(1):23-30.

Eggan K, Akutsu H, Loring J, Jackson-Grusby L, Klemm M, Rideout WM 3rd, Yanagimachi R, Jaenisch R.
Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation.
Proc Natl Acad Sci U S A. 2001 May 22;98(11):6209-14.

Humpherys D, Eggan K, Akutsu H, Hochedlinger K, Rideout WM 3rd, Biniszkiewicz D, Yanagimachi R, Jaenisch R.
Epigenetic instability in ES cells and cloned mice.
Science. 2001 Jul 6;293(5527):95-7.

Rideout WM 3rd, Eggan K, Jaenisch R.
Nuclear cloning and epigenetic reprogramming of the genome.
Science. 2001 Aug 10;293(5532):1093-8.

McKinnell RG.
Cloning of Homo sapiens? No!
Differentiation. 2002 Jan;69(4-5):150-3.

Renard JP, Zhou Q, LeBourhis D, Chavatte-Palmer P, Hue I, Heyman Y, Vignon X.
Nuclear transfer technologies: between successes and doubts.
Theriogenology. 2002 Jan 1;57(1):203-22.

Perry AC, Wakayama T.
Untimely ends and new beginnings in mouse cloning.
Nat Genet. 2002 Mar;30(3):243-4.

Ogonuki N, Inoue K, Yamamoto Y, Noguchi Y, Tanemura K, Suzuki O, Nakayama H, Doi K, Ohtomo Y, Satoh M, Nishida A, Ogura A.
Early death of mice cloned from somatic cells.
Nat Genet. 2002 Mar;30(3):253-4.

Schatten G, Prather R, Wilmut I.
Cloning claim is science fiction, not science.
Science. 2003 Jan 17;299(5605):344.

Hochedlinger K, Jaenisch R.
Nuclear transplantation, embryonic stem cells, and the potential for cell therapy.
N Engl J Med. 2003 Jul 17;349(3):275-86.
 
 

Artificial twins / gemelación artificial

Chan AW, Dominko T, Luetjens CM, Neuber E, Martinovich C, Hewitson L, Simerly CR, Schatten GP.
Clonal propagation of primate offspring by embryo splitting.
Science. 2000 Jan 14;287(5451):317-9.
 

HUMAN (& PRIMATE) STEM CELLS (células troncales humanas y de primates)
 
 

Pioneer papers / Publicaciones pioneras

Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, SwiergielJJ, Marshall VS, Jones JM.
Embryonic stem cell lines derived from human blastocysts.
Science. 1998 Nov 6;282(5391):1145-7.

Shamblott MJ, Axelman J, Wang S, Bugg EM, Littlefield JW, Donovan PJ,Blumenthal PD, Huggins GR, Gearhart JD.
Derivation of pluripotent stem cells from cultured human primordial germ cells.
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13726-31.

Thomas P. Zwaka & James A. Thomson
Homologous recombination in human embryonic stem cells
Nature Biotechnology. Published online: 10 February 2003, doi:10.1038/nbt788
 

Reviews / Revisiones

Thomson JA, Marshall VS.
Primate embryonic stem cells.
Curr Top Dev Biol. 1998;38:133-65.

Gearhart J.
New potential for human embryonic stem cells.
Science. 1998 Nov 6;282(5391):1061-2.

Solter D, Gearhart J.
Putting stem cells to work.
Science. 1999 Mar 5;283(5407):1468-70

Edwards BE, Gearhart JD, Wallach EE.
The human pluripotent stem cell: impact on medicine and society.
Fertil Steril. 2000 Jul;74(1):1-7.

Thomson JA, Odorico JS.
Human embryonic stem cell and embryonic germ cell lines.
Trends Biotechnol. 2000 Feb;18(2):53-7.

Pera MF, Reubinoff B, Trounson A.
Human embryonic stem cells.
J Cell Sci. 2000 Jan;113 ( Pt 1):5-10.

Marshall VS, Waknitz MA, Thomson JA.
Isolation and maintenance of primate embryonic stem cells.
Methods Mol Biol. 2001;158:11-8.

Robertson JA.
Human embryonic stem cell research: ethical and legal issues.
Nat Rev Genet. 2001 Jan;2(1):74-8.

Hoffman LM, Carpenter MK.
Characterization and culture of human embryonic stem cells.
Nat Biotechnol. 2005 Jun;23(6):699-708.
 

Pluripotency and differentiation / Pluripotencia y diferenciación

Amit M, Carpenter MK, Inokuma MS, Chiu CP, Harris CP, Waknitz MA, Itskovitz-Eldor J, Thomson JA.
Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture.
Dev Biol. 2000 Nov 15;227(2):271-8.

Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A.
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
Nat Biotechnol. 2000 Apr;18(4):399-404.

Itskovitz-Eldor J, Schuldiner M, Karsenti D, Eden A, Yanuka O, Amit M, Soreq H, Benvenisty N.
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Mol Med. 2000 Feb;6(2):88-95.

Schuldiner M, Yanuka O, Itskovitz-Eldor J, Melton DA, Benvenisty N.
Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells.
Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11307-12.

Shamblott MJ, Axelman J, Littlefield JW, Blumenthal PD, Huggins GR,Cui Y, Cheng L, Gearhart JD.
Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro.
Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):113-8.

Odorico JS, Kaufman DS, Thomson JA.
Multi lineage differentiation from human embryonic stem cell lines.
Stem Cells. 2001;19(3):193-204.

Strauer BE, Brehm M, Zeus T, Gattermann N, Hernandez A, Sorg RV, Kogler G, Wernet P.
[Intracoronary, human autologous stem cell transplantation for myocardial regeneration following myocardial infarction]
Dtsch Med Wochenschr. 2001 Aug 24;126(34-35):932-8.

Jose B. Cibelli; Ann A. Kiessling; Kerrianne Cunniff; Charlotte Richards; Robert P. Lanza; Michael D. West
Somatic Cell Nuclear Transfer in Humans: Pronuclear and Early Embryonic Development(PDF article)
Journal of Regenerative Medecine. 2001. Nov 26; 2: 25-31.

Cibelli JB, Grant KA, Chapman KB, Cunniff K, Worst T, Green HL, Walker SJ, Gutin PH, Vilner L, Tabar V, Dominko T, Kane J, Wettstein PJ, Lanza RP, Studer L, Vrana KE, West MD.
Parthenogenetic stem cells in nonhuman primates.
Science. 2002 Feb 1;295(5556):819.

Holden C.
Stem cell research. Primate parthenotes yield stem cells.
Science. 2002 Feb 1;295(5556):779-80.

Seydel C.
Stem cell research. Stem cells may shore up transplanted hearts.
Science. 2002 Jan 11;295(5553):253-4.

Woo Suk Hwang, Young June Ryu, Jong Hyuk Park, Eul Soon Park, Eu Gene Lee, Ja Min Koo, Hyun Yong Chun, Byeong Chun Lee, Sung Keun Kang, Sun Jong Kim, Curie Ahn, Jung Hye Hwang, Ky Young Park, Jose B. Cibelli,  Shin Yong Moon
Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst
Science (Published online February 12, 2004)
Atención: visitar la web de Science para la última información sobre la validez y veracidad de este artículo

Artículo eliminado por fraude científico (retracted): último comunicado de Science (12 Enero 2006), cerrando esta controversia.

Vogel G.
Human Embryos Cloned for Stem Cells
Science NOW (12 Feb 2004)

Woo Suk Hwang, Sung Il Roh, Byeong Chun Lee, Sung Keun Kang, Dae Kee Kwon, Sue Kim, Sun Jong Kim, Sun Woo Park, Hee Sun Kwon, Chang Kyu Lee, Jung Bok Lee, Jin Mee Kim, Curie Ahn, Sun Ha Paek, Sang Sik Chang, Jung Jin Koo, Hyun Soo Yoon, Jung Hye Hwang, Youn Young Hwang, Ye Soo Park, Sun Kyung Oh, Hee Sun Kim, Jong Hyuk Park, Shin Yong Moon, Gerald Schatten
Patient-Specific Embryonic Stem Cells Derived from Human SCNT Blastocysts
Science Express Published online 19 May 2005  [DOI: 10.1126/science.1112286]
Atención: visitar la web de Science para la última información sobre la validez y veracidad de este artículo
News from Nature (29 Dec 2005). Timeline of the controversy (from Nature)

Artículo eliminado por fraude científico (retracted): último comunicado de Science (12 Enero 2006), cerrando esta controversia.

Gretchen Vogel
Korean Team Speeds Up Creation Of Cloned Human Stem Cells
Science, Vol 308, Issue 5725, 1096-1097 , 20 May 2005

Boyer LA, Lee TI, Cole MF, Johnstone SE, Levine SS, Zucker JP, Guenther MG, Kumar RM, Murray HL, Jenner RG, Gifford DK, Melton DA, Jaenisch R, Young RA.
Core transcriptional regulatory circuitry in human embryonic stem cells.
Cell. 2005 Sep 23;122(6):947-56.

induced pluripotent human stem cells/ células troncales humanas pluripotentes inducidas

Junying Yu, Maxim A. Vodyanik, Kim Smuga-Otto, Jessica Antosiewicz-Bourget, Jennifer L. Frane, Shulan Tian, Jeff Nie, Gudrun A. Jonsdottir, Victor Ruotti, Ron Stewart, Igor I. Slukvin, James A. Thomson

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Science. 2007 www.sciencexpress.org / 20 November 2007 / Page 1 / 10.1126/science.1151526

Kazutoshi Takahashi, Koji Tanabe, Mari Ohnuki, Megumi Narita, Tomoko Ichisaka, Kiichiro Tomoda, and Shinya Yamanaka

Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors

Cell. 2007 doi:10.1016/j.cell.2007.11.019

Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, Okita K, Mochiduki Y, Takizawa N, Yamanaka S.

Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.

Nat Biotechnol. 2008 Jan;26(1):101-6. Epub 2007 Nov 30.

Park IH, Zhao R, West JA, Yabuuchi A, Huo H, Ince TA, Lerou PH, Lensch MW, Daley GQ.

Reprogramming of human somatic cells to pluripotency with defined factors.

Nature. 2008 Jan 10;451(7175):141-6. Epub 2007 Dec 23.

MOUSE STEM CELLS (células troncales de ratón) 
 

Pioneer papers / Publicaciones pioneras
 

Evans MJ, Kaufman MH.
Establishment in culture of pluripotential cells from mouse embryos.
Nature. 1981 Jul 9;292(5819):154-6.

Martin GR.
Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634-8.

Matsui Y, Zsebo K, Hogan BL.
Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture.
Cell. 1992 Sep 4;70(5):841-7.

Reviews and features / Revisiones y características

Bradley A, Robertson E.
Embryo-derived stem cells: a tool for elucidating the developmental genetics of the mouse.
Curr Top Dev Biol. 1986;20:357-71.

Robertson E.
Pluripotential stem cell lines as a route into the mouse germ line
Trends Genet 1986, 2: 9-13.

Smith AG.
Mouse embryo stem cells: their identification, propagation and manipulation.
Semin Cell Biol. 1992 Dec;3(6):385-99. R

Keller GM.
In vitro differentiation of embryonic stem cells.
Curr Opin Cell Biol. 1995 Dec;7(6):862-9.

Robertson EJ.
Derivation and maintenance of embryonic stem cell cultures.
Methods Mol Biol. 1997;75:173-84.

Gardner RL, Brook FA.
Reflections on the biology of embryonic stem (ES) cells.
Int J Dev Biol. 1997 Apr;41(2):235-43.

Brook FA, Gardner RL.
The origin and efficient derivation of embryonic stem cells in the mouse.
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5709-12.

Dani C, Chambers I, Johnstone S, Robertson M, Ebrahimi B, Saito M, Taga T, Li M, Burdon T, Nichols J, Smith A.
Paracrine induction of stem cell renewal by LIF-deficient cells: a new ES cell regulatory pathway.
Dev Biol. 1998 Nov 1;203(1):149-62.

Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Scholer H, Smith A.
Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
Cell. 1998 Oct 30;95(3):379-91.

Raz R, Lee CK, Cannizzaro LA, d'Eustachio P, Levy DE.
Essential role of STAT3 for embryonic stem cell pluripotency.
Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2846-51.

Blau HM, Brazelton TR, Weimann JM.
The evolving concept of a stem cell: entity or function?
Cell. 2001 Jun 29;105(7):829-41.

Soria B, Skoudy A, Martin F.
From stem cells to beta cells: new strategies in cell therapy of diabetes mellitus.
Diabetologia. 2001 Apr;44(4):407-15.
 

mouse ES cells and KO mice / células ES de ratón y ratones KO

Robertson E, Bradley A, Kuehn M, Evans M.
Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector.
Nature. 1986 Oct 2-8;323(6087):445-8.

Thomas KR, Capecchi MR.
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.
Cell. 1987 Nov 6;51(3):503-12.

Robertson EJ.
Using embryonic stem cells to introduce mutations into the mouse germ line.
Biol Reprod. 1991 Feb;44(2):238-45.

Fedorov LM, Haegel-Kronenberger H, Hirchenhain J.
A comparison of the germline potential of differently aged ES cell lines and their transfected descendants.
Transgenic Res. 1997 May;6(3):223-31.

Udy GB, Parkes BD, Wells DN.
ES cell cycle rates affect gene targeting frequencies.
Exp Cell Res. 1997 Mar 15;231(2):296-301.
 

mouse ES cells and in vivo differentiation / células ES de ratón y diferenciación in vivo

Nagy A, Rossant J, Nagy R, Abramow-Newerly W, Roder JC.
Derivation of completely cell culture-derived mice from early-passage embryonic stem cells.
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8424-8.

Brustle O, Jones KN, Learish RD, Karram K, Choudhary K, Wiestler OD, Duncan ID, McKay RD. Related Articles
Embryonic stem cell-derived glial precursors: a source of myelinating transplants.
Science. 1999 Jul 30;285(5428):754-6.

Fairchild PJ, Brook FA, Gardner RL, Graca L, Strong V, Tone Y, Tone M, Nolan KF, Waldmann H.
Directed differentiation of dendritic cells from mouse embryonic stem cells.
Curr Biol. 2000 Nov 30;10(23):1515-8.

Liu S, Qu Y, Stewart TJ, Howard MJ, Chakrabortty S, Holekamp TF, McDonald JW.
Embryonic stem cells differentiate into oligodendrocytes and myelinate in culture and after spinal cord transplantation.
Proc Natl Acad Sci U S A. 2000 May 23;97(11):6126-31.

Kim JH, Auerbach JM, Rodriguez-Gomez JA, Velasco I, Gavin D, Lumelsky N, Lee SH, Nguyen J, Sanchez-Pernaute R, Bankiewicz K, McKay R.
Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease.
Nature 2002 Jul 4;418(6893):50-6
 

mouse ES cells and in vitro differentiation / células ES de ratón y diferenciación in vitro

Soria B, Roche E, Berna G, Leon-Quinto T, Reig JA, Martin F.
Insulin-secreting cells derived from embryonic stem cells normalize glycemia in streptozotocin-induced diabetic mice.
Diabetes. 2000 Feb;49(2):157-62.
 

adult somatic stem cells / células troncales somáticas de adultos

Bjornson CR, Rietze RL, Reynolds BA, Magli MC, Vescovi AL.
Turning brain into blood: a hematopoietic fate adopted by adult neural stem cells in vivo.
Science. 1999 Jan 22;283(5401):534-7.

Jackson KA, Mi T, Goodell MA.
Hematopoietic potential of stem cells isolated from murine skeletal muscle.
Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14482-6.

Mezey E, Chandross KJ, Harta G, Maki RA, McKercher SR.
Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow.
Science. 2000 Dec 1;290(5497):1779-82.

Kondo T, Raff M.
Oligodendrocyte precursor cells reprogrammed to become multipotential CNS stem cells.
Science. 2000 Sep 8;289(5485):1754-7.

Fuchs E, Segre JA.
Stem cells: a new lease on life.
Cell. 2000 Jan 7;100(1):143-55.

Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson SM, Li B, Pickel J, McKay R, Nadal-Ginard B, Bodine DM, Leri A, Anversa P.
Bone marrow cells regenerate infarcted myocardium.
Nature. 2001 Apr 5;410(6829):701-5.

McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.
Muscle-derived hematopoietic stem cells are hematopoietic in origin.
Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1341-1346.

Ying QL, Nichols J, Evans EP, Smith AG.
Changing potency by spontaneous fusion.
Nature. 2002 Apr 4;416(6880):545-8.

Terada N, Hamazaki T, Oka M, Hoki M, Mastalerz DM, Nakano Y, Meyer EM, Morel L, Petersen BE, Scott EW.
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion.
Nature. 2002 Apr 4;416(6880):542-5.

Wurmser AE, Gage FH.
Stem cells: Cell fusion causes confusion.
Nature. 2002 Apr 4;416(6880):485-7.

Wells WA.
Is transdifferentiation in trouble?
J Cell Biol. 2002 Apr 1;157(1):15-8. Review.

Morshead CM, Benveniste P, Iscove NN, van der Kooy D.
Hematopoietic competence is a rare property of neural stem cells that may depend on genetic and epigenetic alterations.
Nat Med. 2002 Mar;8(3):268-73.

D'Amour KA, Gage FH.
Are somatic stem cells pluripotent or lineage-restricted?
Nat Med. 2002 Mar;8(3):213-4.

Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, Reyes M, Lenvik T, Lund T, Blackstad M, Du J, Aldrich, S, Lisberg A, Low WC, Largaespada DA, Verfaillie CM.
Pluripotency of mesenchymal stem cells derived from adult marrow.
Nature 2002 Jul 4;418(6893):41-9

Avots A, Harder F, Schmittwolf C, Petrovic S, Muller AM.
Plasticity of hematopoietic stem cells and cellular memory.
Immunol Rev. 2002 Sep;187:9-21.

Daley G.
Prospects for stem cell therapeutics: myths and medicines.
Curr Opin Genet Dev. 2002 Oct;12(5):607.

Strom T, Field L, Ruediger M.
Allogeneic stem cells, clinical transplantation and the origins of regenerative medicine.
Curr Opin Immunol. 2002 Oct;14(5):601.

Lanza R, Moore MA, Wakayama T, Perry AC, Shieh JH, Hendrikx J, Leri A, Chimenti S, Monsen A, Nurzynska D, West MD, Kajstura J, Anversa P.
Regeneration of the Infarcted Heart With Stem Cells Derived by Nuclear Transplantation.
Circ Res. 2004 Feb 5 [Epub ahead of print]

mouse ES cells and cloning / células ES de ratón y clonación

Munsie MJ, Michalska AE, O'Brien CM, Trounson AO, Pera MF, Mountford PS.
Isolation of pluripotent embryonic stem cells from reprogrammed adult mouse somatic cell nuclei.
Curr Biol. 2000 Aug 24;10(16):989-92.

Wakayama T, Tabar V, Rodriguez I, Perry AC, Studer L, Mombaerts P.
Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer.
Science. 2001 Apr 27;292(5517):740-3.

Rideout, W.M. III, Hochedlinger, K., Kyba, M. Daley, G., and Jaenisch, R.
Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy.
Cell. 2002 Apr 5;109(1):17-27.

Kyba, M., Perlingeiro, R.C.R., and Daley G.Q.
HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors.
Cell. 2002 Apr 5;109(1):29-37.

Hubner K, Fuhrmann G, Christenson LK, Kehler J, Reinbold R, De La Fuente R, Wood J, Strauss JF 3rd, Boiani M, Scholer HR.
Derivation of oocytes from mouse embryonic stem cells.
Science. 2003 May 23;300(5623):1251-6. Epub 2003 May 01.

Meissner A, Jaenisch R.
Generation of nuclear transfer-derived pluripotent ES cells from cloned Cdx2-deficient blastocysts.
Nature. 2005 Oct 16; [Epub ahead of print]

Chung Y, Klimanskaya I, Becker S, Marh J, Lu SJ, Johnson J, Meisner L, Lanza R.
Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres.
Nature. 2005 Oct 16; [Epub ahead of print]

induced pluripotent mouse stem cells/ células troncales de ratón pluripotentes inducidas

Takahashi K, Yamanaka S.

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.

Cell. 2006 Aug 25;126(4):663-76.

Wernig M, Meissner A, Foreman R, Brambrink T, Ku M, Hochedlinger K, Bernstein BE, Jaenisch R.

In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state.

Nature. 2007 Jul 19;448(7151):318-24.

Okita K, Ichisaka T, Yamanaka S.

Generation of germline-competent induced pluripotent stem cells.

Nature. 2007 Jul 19;448(7151):313-7.

Takahashi K, Okita K, Nakagawa M, Yamanaka S.

Induction of pluripotent stem cells from fibroblast cultures.

Nat Protoc. 2007;2(12):3081-9.

Meissner A, Wernig M, Jaenisch R.

Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells.

Nat Biotechnol. 2007 Oct;25(10):1177-81. Epub 2007 Aug 27.

Hanna J, Wernig M, Markoulaki S, Sun CW, Meissner A, Cassady JP, Beard C, Brambrink T, Wu LC, Townes TM, Jaenisch R.

Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin.

Science. 2007 Dec 21;318(5858):1920-3. Epub 2007 Dec 6.

Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, Okita K, Mochiduki Y, Takizawa N, Yamanaka S.

Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.

Nat Biotechnol. 2008 Jan;26(1):101-6. Epub 2007 Nov 30.

Aoi T, Yae K, Nakagawa M, Ichisaka T, Okita K, Takahashi K, Chiba T, Yamanaka S.

Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells.

Science. 2008 Feb 14;

Yamanaka S.

Induction of pluripotent stem cells from mouse fibroblasts by four transcription factors.

Cell Prolif. 2008 Feb;41 Suppl 1:51-6.

LIBROS e INFORMES

Stem Cell Biology
Marshak D.R., Gardner R.L., Gootlieb D. (eds.)
Col Spring Harbor Laboratory Press (2001)
Cold Spring Harbor, New York
ISBN 0-87969-575-7

Gene Targeting: A practical approach
Joyner A. L. (ed.) 2nd. edition (2000)
Oxford University Press, Oxford
ISBN 0-19-963792-X

The Second Creation: Dolly and the age of biological control
Wilmut I., Campbell K., Tudge C.
Farrar, Straus and Giroux Publishers / Harward University Press (2000)
ISBN 0-674-00586-4

Informe sobre clonación: En las fronteras de la vida.
Comité de Expertos sobre Bioética y Clonación, Madrid, 1999
Insituto de Bioética
Fundación de Ciencias de la Salud
 

HANDBOOK OF STEM CELLS, TWO-VOLUME SET WITH CD-ROM, 1-2
Volume 1-Embryonic Stem Cells; Volume 2-Adult & Fetal Stem Cells
Robert Lanza et al.
Academic Press (Elsevier) 2004

EMPRESAS

Geron

Advanced Cell Technologies

ES Cell International

Stem Cells Inc.

Infigen

Agrobiogen

Bioprotein

Lazaron

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Instituciones, Medios de comunicación y WEBs relacionadas

Subdirección General de Investigación en Terapia Celular y Medicina Regenerativa (Instituto de Salud Carlos III)

Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER)

Centro de Medicina Regenerativa de Barcelona (CMRB)
 

Stem Cell Research (Daily Updated News - YAHOO)

Stem cells Network

Instituto ROSLIN (Edimburgo, Escocia)

Rudolf Jaenisch's Laboratory (Whitehead Institute, MIT, USA)

Teruhiko Wakayama's Laboratory (CDB, Kobe, Japan)

Anthony Perry's Laboratory (CDB, Kobe, Japan)

Instituto Valenciano de Infertilidad

Institut Universitari Dexeus (Barcelona)