|
|
Dr. Lluís Montoliu Research Scientist CSIC Departament of Molecular and Cellular Biology National Centre of Biotechnology (CNB-CSIC) Campus de Cantoblanco, C/ Darwin 3 28049 Madrid, Spain
Tel. +34 91 585-4844 (office) / +34 91 585-4530 (laboratory) Fax. +34 91 585-4506 / skype: lluis.montoliu / Twitter: LluisMontoliu / also in Facebook and LinkedIn e-mail:
montoliu@cnb.csic.es
|
|||
|
|
Congenital defects and development disorders Research Group: Animal Models by Genetic Manipulation
Research Summary We are interested in understanding how mammalian expression domains work and how they are organised within genomes. We would like to know the required regulatory elements that identify a given expression domain and specify its expression pattern in space, time and level, to improve the dessign of gene transfer strategies, used for animal transgenesis and for gene therapy.
We use two experimental models: the mouse
tyrosinase and whey acidic protein genes, two independent
developmentally regulated and tissue-specific loci that have served us
to identify a number of
Further, our laboratory generates and analyses new animal models of human diseases by genetic modification of mice. We have used a number of tyrosinase transgenic mice to address the retinal deficits commonly associated with albinism, the normal mammalian retinal development and the genes involved in the process. Finally, through collaborations, we have a number of additional animal models (transgenics and knockouts) for CNS-related diseases or conditions (Alzheimer, pain, psychosis).
From January 1, 2009, CNB has become the Spanish node of the European Project EMMA.
Browse publications by Lluís Montoliu
Dr. Lluís Montoliu's Laboratory
(PDF documents)
Genetic differences among C57BL/6 substrains
to the private zone of this WEB
|
Molecular Biosciences (UAM) Course 2011-2012
Master in "Molecular and Cellular Biology" Master in "Molecular Biomedicine" Master in "Biotechnology"
Module BM5: Genomics, Proteomics & Genetic Modification 26 September - 4 November 2011
Previous post-graduate courses (1998-2011) Bioinformatic Resources (2005)
WEB and e-mail: histologia@cnb.csic.es Tel. +34 91585-5349 / 4903 NEW WEB - EMMA PROJECT
Mouse Embryo Cryopreservation Facility of CNB
WEB and e-mail: criocnb@cnb.csic.es Tel. +34 91585-5349 / 4312
Baiyun International Convention Center, Guangzhou/Canton, PR China (25-27 February 2013)
Protocols for the generation and analysis of transgenic mice
Direct download of MENDEL Excel workbook
Laboratory protocols (restricted)
Advanced Protocols for Animals Transgenesis. An ISTT Manual S. Pease & T. Saunders (Eds.) - Springer 2011
PhD thesis developed in Dr. Lluís Montoliu's laboratory
Database of transgenic mice, by Lluís Montoliu (2003)
Press here to request a free copy for academic-only use
"Generation of Transgenic Mice" by Lluís Montoliu (1997)
Press here to request a free copy for academic-only use
Information on albinism for scientists and for persons with albinism
Albinismo. Una condición genética, dos realidades: España y Senegal
Meetings, congresses, conferences and workshops organised
Legislación Europea, Nacional y Autonómica sobre protección de los animales utilizados para la experimentacion y otros fines cientificos y sobre el uso de organismos modificados genéticamente CSIC Serrano 113 (1st floor) 28006 Madrid, Spain Tel. 915681494 email: subcomitedebioetica@csic.es
How to reach the CNB?
(Location of CNB at the Campus of the UAM) Pictures of CNB
|
||
key
regulatory elements, including boundaries and LCRs. We address our
experiments in vivo, using transgenic mice carrying large chromosomal-
type constructs that we engineer by homologous recombination to
investigate the functional role of specific sequences, and in vitro,
using cells and chromatin DNA-protein analyses.
Annual
research reports CNB
