CNB Histology Facility
Centro Nacional de Biotecnología (CNB) Consejo Superior de Investigaciones Científicas (CSIC) Campus de Excelencia UAM+CSIC

 

telephone: 915854903
histologia@cnb.csic.es

 

CNB Histology Facility

 

Time-table for contacting/receiving/delivering samples:

from 08:00 to 15:00 hours

 

Centro Nacional de Biotecnología

Departament of Molecular and Cellular Biology

(Laboratory 126)

Campus de Cantoblanco, C/ Darwin 3

28049 Madrid

.

Responsible Scientist: Lluís Montoliu (tel. 915854844, laboratory 111)
Technician Specialist: Soledad Montalbán (tel. 915855349-office 128; 915854903-laboratory 126)
Supporting Technician: Oscar Sánchez (tel. 915854903-laboratory 126; 915854312-office B15)

The CNB Histology Facility is integrated within the Scientific-Technological Platform INNOTEK, in support of research,

belonging to the International Campus of Excellence UAM+CSIC.

 

CNB Histology Facility Report (2009-2010)

Presentation of CNB Histology Facility (8 March 2012)

Histology Facility (Feb. 2011) - Pictures: Inés Poveda

 


Wax/Paraffin                                                       Frozen (Cryostat)                      Plastic/resin (Historesin)

Our Forms

Our Expertise

You can browse this table to see the species, tissues and organs we have proccessed to date

Our Fees

last update for these fees:  January 2007

 
Service
Unit
Fee per unit

(CNB-INNOTEK user)*

€
Fee per unit 

(other CSIC centres)

€

 Fee per unit
(other public research institutions, not CSIC)
€

+18 % VAT
not included 
 

Fee per unit
(private research institutions)
€

+18 % VAT
not included

Preparing a paraffin block
1 U = 1 block
  5 Euros 6 Euros 7,5 Euros 11,5 Euros
Cutting a paraffin block 1 U = 1 block / 10 slides 4,5 Euros 5,4 Euros 7 Euros 10,5 Euros
" 1 U = 1 block / 5 slides 3 Euros 3,6 Euros 4,7 Euros 7 Euros
Staining and mounting (paraffin)
1 U = 10 slides
  4,5 Euros 5,4 Euros 7 Euros 10,5 Euros

"

1 U = 5 slides

  3 Euros 3,6 Euros 4,7 Euros 7 Euros
Preparing a frozen block (cryostat)
1 U = 1 block
  2 Euros 2,5 Euros 3 Euros 4,5 Euros
Cutting a frozen block (cryostat)
1 U = 10 slides
  4,5 Euros 5,4 Euros 7 Euros 10,5 Euros

"

1 U = 5 slides

  3 Euros 3,6 Euros 4,7 Euros 7 Euros
Staining and mounting (frozen)
1 U = 10 slides
  2,5 Euros 3 Euros 4 Euros 6 Euros

"

1 U = 5 slides

  1,5 Euros 2 Euros 2,5 Euros 5 Euros
Preparing a plastic block (Historesin)
1 U = 1 block
  16 Euros 19 Euros 24 Euros 36 Euros
Cutting a plastic block (Historesin)
1 U = 10 slides
  18 Euros 22 Euros 29 Euros 43,5 Euros

"

1 U = 5 slides

  15 Euros 18 Euros 23,5 Euros 35 Euros
Staining and mounting (plastic)
1 U = 10 slides
  1,5 Euro 2 Euros 2,5 Euros 5 Euros

"

1 U = 5 slides

  1 Euro 1,5 Euros 2 Euros 2,5 Euros
Other procedures 
(specific stains, immunohistochemistry, ....)
please inquire
 please inquire please inquire please inquire  please inquire 

(*) Researchers/Users from the platform INNOTEK (CBMSO, CNB, IIBm, UAM) are also included

Important remarks:
1) The requested services by CNB personnel will always have preference for execution before the rest of orders from external users.
2) Unless specifically indicated, all blocks will be prepared and fixed in 4% PFA.
3) Unless specifically indicated, all sections from paraffin and plastic blocks will be obtained at 5 micrometers thickness, and those from frozen blocks at 15 micrometers thickness.
4) We offer two routine basic staining protocols, initially available: hematoxylin/eosin and Cresyl Violet. For other staining procedures, please inquire.
5) If the user needs less slides than indicated in the contracted 1 unit (10 or 5 slides) we will invoice still 1 Unit.

Equipment available

1 motorized Microtome (plastic/resin/paraffin/wax) Leica RM2155

1 motorized Microtome (plastic/resin/paraffin/wax) Thermo Scientific, Shandon, FINESSE ME+

1 Tisue Processing Station Leica TP1020

1 Cryostat Leica CM1900

1 Tissue Embedding Station Leica EG1160

2 thermostatic baths Leica HI1210

Coordination

Since 2009, the CNB Histology Facility is associated with the IIBm-UAM/CSIC Histology Facility. The two centres, participating in INNOTEK, merged their operations under the coordination of the CNB Histology Facility, enabling them to offer CNB and IIB researchers greater processing capacity of histological samples. The CNB Histology Facility has been training personnel from the IIB-UAM/CSIC Histology Facility and supervising work, aiming to establish equivalent output standards.

WEB last updated on: 8 March 2012