Protein array and antibody array services

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Protein arrays and antibody arrays can be used for multiple applications and allow large-scale screening of proteins from cell lysates and to detect protein-protein interactions.

We offer our procedures as a scientific collaboration with those research groups interested in using array-based technologies for three main applications:


1) Protein-protein interactions (PPI). Detection and identification of protein-protein interactions (several thousand proteins may be screened in a single experiment).
2) Differential protein expression (DPE). Antibody arrays are used to bind and detect the levels of proteins present in cell lysates.
3) Antibody specificity profiling (ASP). Screening of the specificity of your antibody of choice against a large pool of human proteins.

Quotations are provided on a custom-dependent basis and mainly depend on the availability of chemical required to carry out the experiments upon request.

Our goal is to provide you with meaningful results using the lowest budget possible, meaning that if we can afford with chemicals already in use in our lab, they will not be charged.

protein array and antibody array services

We currently offer our experimental and bioinformatic platform to researchers and companies interested in these approaches.

Contact us:

Juan Casado-Vela

jcasado@cnb.csic.es

Location: Spanish National Biotechnology Centre (CNB-CSIC).
Darwin 3, Campus de Cantoblanco, 28049 Madrid, Spain.

 

PolyAn

See our General terms and conditions. Terms and conditions

Other services. Outsource laboratory services.

What do you need?

  1. For protein-protein interaction experiments:
  2. For differential protein expression experiments:
  3. For antibody specificity tests: (purified antibody, monoclonal antibodies or polyclonal against known peptides preferably).
Protein arrays and antibody arrays high density protein array for protein-protein interaction screening
example of differential display protein profiling using antibody arrays

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Relevan publications:

  1. Protein arrays: recent achievements and their application to study the human proteome. Current Proteomics. Casado-Vela, J. et al. 2013.
  2. Evaluation of homo- and hetero-functionally activated glass surfaces for Optimized Antibody Array. Analytical Biochemistry. 2014. DOI: http://dx.doi.org/10.1016/j.ab.2014.01.002.
  3. Protein-protein interactions: gene acronym redundancies and current limitations precluding fully automated data integration. Casado-Vela, J. et al. Proteomes. 1(1), 3-24. 2013. Over 1000 downloads.
  4. Lights and shadows of proteomic technologies for the study of protein species including isoforms, splicing variants and protein post-translational modifications. Casado-Vela, J. et al. Proteomics. 2011 Feb;11(4):590-603.
  5. Approaches for the study of cancer: towards the integration of genomics, proteomics and metabolomics. Casado-Vela, J. Clin Transl Oncol. 2011 Sep;13(9):617-28.
  6. Data Analysis Strategies for Protein Microarrays. Microarrays. 2012, 1(2), 64-83.

Full list of antibodies available for cell signaling, apoptosis and neuro-phosphorylation profiling arrays Full list of antibodies for antibady array experiments

Campus map Full list of human proteins
How to get here... Full list of human genes
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Our lab is financed by JAE-Doc Grants «Junta para la Ampliación de Estudios» and co-financed by the European Social Fund.

Featured collaborators and companies

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Webmaster: (jcasado@cnb.csic.es) Last update: March 2014
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