Protein arrays and antibody arrays can be used for multiple applications and allow large-scale screening of proteins from cell lysates and to detect protein-protein interactions.
We offer our procedures as a scientific collaboration with those research groups interested in using array-based technologies for three main applications:
1) Protein-protein interactions (PPI). Detection and identification of protein-protein interactions (several thousand proteins may be screened in a single experiment).
2) Differential protein expression (DPE). Antibody arrays are used to bind and detect the levels of proteins present in cell lysates.
3) Antibody specificity profiling (ASP). Screening of the specificity of your antibody of choice against a large pool of human proteins.
Quotations are provided on a custom-dependent basis and mainly depend on the availability of chemical required to carry out the experiments upon request.
Our goal is to provide you with meaningful results using the lowest budget possible, meaning that if we can afford with chemicals already in use in our lab, they will not be charged.
We currently offer our experimental and bioinformatic platform to researchers and companies interested in these approaches.
Scanning, digital image analysis service, bioinformatic analysis of the results.
Data normalization by reference (housekeeping) proteins.
Dye Swapping:
if two different samples are labeled with different dyes (Cy3 and Cy5) it is advisable to carry out reverse labeling to preclude label-specific differences.