We are interested in understanding how plants are able to perceive changes in their environmentand integrate stress signals with their internal developmental programs, to induce adaptive responses and survive in nature. This integration depends on complex signalling networks that regulate the genetic reprogramming of the cell. The main focus of my lab is to understand one of the pathways involved in this network, the jasmonate(JA) signalling pathway, in Arabidopsis thaliana.
Gimenez-Ibanez S, Boter M, Fernández-Barbero G, Chini A, Rathjen JP, Solano R. The Bacterial Effector HopX1 Targets JAZ Transcriptional Repressors to Activate Jasmonate Signaling and Promote Infection in Arabidopsis. PLoS Biology 2014; 12:e1001792
Franco-Zorrilla JM, López-Vidriero I, Carrasco JL, Godoy M, Vera P, Solano R. DNA-binding specificities of plant transcription factors and their potential to define target genes. Proc Natl Acad Sci USA; 2014; 111:2367-2372
Monte I, Hamberg M, Chini A, Gimenez-Ibanez S, García-Casado G, Porzel A, Pazos F, Boter M, Solano R. Rational design of a ligand-based antagonist of jasmonate perception. Nat Chem Biol 2014; 10:671-676
Chico JM, Fernández-Barbero G, Chini A, Fernández-Calvo P, Díez-Díaz M, Solano R. Repression of Jasmonate-Dependent Defenses by Shade Involves Differential Regulation of Protein Stability of MYC Transcription Factors and Their JAZ Repressors in Arabidopsis. Plant Cell 2014; 26:1967-1980
Boer DR, Freire-Rios A, van den Berg WAM, Saaki T, Manfield IW, Kepinski S, López-Vidrieo I, Franco-Zorrilla JM, de Vries SC, Solano R, Weijers D, Coll M. Structural Basis for DNA Binding Specificity by the Auxin-Dependent ARF Transcription Factors. Cell 2014; 156:577-589
Plants are able to perceive changes in their environment and integrate stress signals with their internal developmental programs to induce adaptive responses and survive in nature. This integration depends on complex signalling networks that regulate the genetic re-programming of the cell. The main focus in my lab is to understand one of the pathways involved in this network, the jasmonate (JA) signalling pathway in Arabidopsis thaliana. JA are fatty acid-derived signalling molecules essential for plant survival in nature, since they are important activators of stress responses and developmental programs. We aim to identify the components of this pathway and understand how these components explain the molecular interactions of the JA pathway with other pathways within the network. Understanding these molecular interactions is essential to decipher how one single hormone can activate so many different physiological responses in the plant, and how the plant is able to discriminate between different stresses and select the correct set of responses to each. This knowledge is basic to design biotechnological and agronomic applications.
The major achievements of our group in the last two years are:
- Identification and characterisation of the bacterial effector HopX1, which enhances plant susceptibility to biotrophic pathogens by activating the JA signalling pathway. HopX1 has a protease activity that degrades the key JA-repressors JAZ (Gimenez-Ibanez et al. PLoS Biol 2014)
- Design and characterisation of a potent, specific antagonist of JA perception, COR-MO, with important biotechnological potential (Monte et al. Nat Chem Biol 2014)
- Determination of the DNA-binding sequence specificities of over 60 plant transcription factors, through the use of a protein-binding-microarray (Franco-Zorrilla et al. Proc Natl Acad Sci USA 2014; Boer et al. Cell 2014)
- Discovery of the mechanism by which canopy shade reduces JA-dependent defences (Chico et al. Plant Cell 2014)
- Identification and characterisation of three bHLH transcription factors that repress JA responses (Fonseca et al. PLoS One 2014)
- Discovery of the mechanism by which MYC transcription factors (MYC2, 3 and 4) regulate glucosinolate biosynthesis (Schweizer et al. Plant Cell 2013)
- Analysis of protein disorder in the Arabidopsis thaliana proteome (Pietrosemoli et al. PLoS One, 2013)