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Sequence analysis and protein structure prediction |
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SERVICE DESCRIPTION
 Sequence analysis and protein structure prediction using bioinformatic methods are two approaches that complement and in some cases simplify experimental work. These approaches can be applied to multiple areas of research. Using this type of analysis, and starting from the primary sequence of a gene, it is possible to obtain data on evolution, functional/structural domain organization, functionally relevant regions or residues, functional hypothesis, possible subcellular localisation, interactions with other genes, structural models, etc...
The main function of the service is to provide bioinformatic support in sequence analysis and protein structure prediction to all experimental groups at the CNB that might require it. This includes, among other things:
- Localization of homologous genes within different genomes, totally or partially sequenced, using high sensibility methods
- Generation of high quality multiple sequence alignments
- Evolution: gene distribution within organisms/kingdoms, phylogenetic tree generation...
- Subcellular localization prediction
- Structural/functional domains localization
- Prediction of functionally relevant residues or regions
- Prediction of post-translational modification sites (phosphorylation, glycosylation,..)
- Prediction of interaction sites
- Prediction of functional relationships with other genes by cotext methods (not based on homology) - Prediction of physical/functional interactions
- Generation of structural organisation drafts: localization of globular domain, unstructured regions, coiled-coil, transmembrane regions, etc...
- Secondary structure prediction
- 3D structure prediction for close and remote homology
Generation of high-quality structural representations for publication.
Additionally, the service organizes in collaboration with other CNB services, periodic courses and seminars about bioinformatic approaches for sequence analysis and protein structure prediction.
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