The CNB Proteomics Facility (created in 1999) aims to develop a technological platform suitable for large-scale protein identification and characterization, offering its services to the CNB scientific community as well as to external researchers.
Massive protein identification and characterization is performed through multidimensional nano-HPLC chromatography coupled to either a nano-electrospray ion trap or to a MALDI TOF/TOF mass spectrometer (LC-MS/MS). Comparison between complex proteomes (differential proteomics) is done through the analysis of fluorescent-labeled samples and differential 2D-electrophoresis followed by software-aided sample analysis (2D-DIGE) as well as through the use of isotope stable tags (ICPL, SILAC, iTRAQ) in combination with LC-MS/MS. SELDI-ToF MS (Surface enhanced laser desorption-ionization-time of flight mass spectrometry) is used to obtain protein expression profiles.
For educational purposes, we organize practical courses on topics such as Quantitative Proteomics or bioinformatics. Dr Albar is also the General Coordinator of Proteored, the Spanish National Institute for Proteomics.
