|
|
Head of service:
Mª del Carmen Moreno-Ortiz Navarro
|
Contact
|
Personnel:
Sara Escudero García
Pablo García Bravo
Sonia Rodríguez Murcia
Almudena González García
Website: Servicio de Citometría de Flujo
|
|
|
SERVICE DESCRIPTION
 Flow Cytometry (CMF) is a multiparametric cell
analysis technique whose purpose is to detect
and identify molecules and cell structures using
fluorescent markers and conjugated antibodies.
Flow sorting is an extension of this technology, by which any
cell or object measured can be separated selectively from
the suspension based on properties measured in flow.
The
facility provides scientific and technological support to the different
CNB research groups that require it, for which have the
technical and human means suitable to guarantee maximum
quality and continuous improvement of all our services.
The facility offers:
• Training and advice on the principles
and applications of analytical flow
cytometry, to obtain maximum advantage
• Development and optimization new applications incorporating
new technologies and new reagents
• Quantification of secreted cytokines by multiplexed assays
• Result analysis using specialised software packages
• Cell isolation by cell sorting (sorting of cell populations
including cell suspensions derived from any animal organ
as well as from cell lines
|
 Equipment:
• BD FACSCalibur analyzer: 4 colours, 2 laser excitation
(488 and 633 nm)
• 2 Beckman Coulter EPICS XL-MCL analyzers: 4 colours,
1 laser excitation (488 nm)
• Beckman Coulter Cytomics FC500 analyzer: 5 colours, 2
laser excitation (488, 633 nm)
• BD LSRII analyzer: 8 colors, 3 laser excitation (488, 633
and 405 nm)
• Beckman Coulter Gallios analyzer: 10 colours, 3 laser
excitation (488, 633, 405 nm)
• Luminex 100 IS multiparametric analyzer: a system that
can be used to quantify multiple cytokines (up to 100) or
any other soluble molecule from a single sample
• Cell Sorter Beckman Coulter EPICS-ALTRA Hypersort
The unit also provides the computer science system to
analyze the results obtained: 1 Mac and 2 PC platforms
running specialised software packages (Cell
Quest (Mac), WindMDI (PC), CXP (PC), MultiTime
(PC), MultiCycle (PC), DIVA (PC), FlowJo (PC),
Summit (PC), Kaluza (PC), etc. |
.
|
