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Head of service:
Cristina Patiño Martín
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Contact
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Personnel:
Javier Bueno Chamorro
Rocío San Andrés Cervilla
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SERVICE DESCRIPTION
 The Electron Microscopy service offers a variety of equipment and techniques for the preparation, processing and analysis of biological samples by transmission electron microscopy.
Techniques offered include chemical fixation and inclusion in epoxy and acrylic resins, cryofixation (plunge-freezing, high pressure freezing), cryosubstitution and inclusion in low-temperature resins, ultramicrotomy, immunogold staining, negative staining, negative immunostaining, in situ hybridization, conventional transmission electron and low electron dose microscopy.
The staff provides support to users both in the application of techniques and for equipment use. We offer regular training in the techniques and methods available. We are also responsible, in cases where required, for sample preparation and image acquisition, and provide support for data interpretation.
The service has the following specialised equipment:
• Jeol JEM-1011 transmission electron microscope with ES1000W Gatan camera
• Leica EM MED 020 carbon coating system
• Leica Ultracut UC6 cryo-ultramicrotome with Leica FC6 cryochamber
• Reichert Ultracut E ultramicrotome
• Leica EM AFS2 automatic cryosubstitution system
• Leica EM PACT 2 instrument for high-pressure vitrification
• Leica EM TRIM sample trimmer (pyramitome)
• Reichert Knifemaker
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