Closer to the end of intestinal infections by Escherichia coli

Microscope image of intestinal mucosa affected by Escherichia coli Microscope image of intestinal mucosa affected by Escherichia coli Luis Ángel Fernández, CNB-CSIC

A study led by scientists from the Centro Nacional de Biotecnología of the CSIC finds fundamental genes for altering the intestinal mucosa. The research is a step toward development of an effective vaccine against Escherichia coli intestinal infections.


A group of researchers led by the Centro Nacional de Biotecnología of the CSIC (CNB-CSIC) has found the key to avoiding the most damaging effects of intestinal infection caused by a strain of the bacterium E. coli.  The scientists observed the colonization capacity of this bacterium in the intestinal mucosa and discovered several features that could be used to develop a vaccine against this type of intestinal infection.

"The pathogenic E. coli strain we studied causes severe diarrhoea in children, with high prevalence in developing countries," says researcher Luis Ángel Fernández, from the CNB-CSIC and senior author of the paper.  Fernández highlighted the importance of the discovery in this study, which has been published in the journal PLoS Pathogens.  The scientists showed that for E. coli to bind to the intestinal mucosa, a group of proteins called effectors are involved in forming a lesion that destroys the intestinal microvilli, essential for nutrient absorption.

The study was carried out using enteropathogenic E. coli strains (EPEC), which are very similar to the enterohaemorrhagic E. coli strains (EHEC) that cause similar lesions and are associated with outbreaks of food poisoning in developed countries.  The vaccines that could be developed might thus protect against EPEC and EHEC strains.

How do the effectors work?

The intestinal mucosa is a defensive barrier against infections.  Intestinal pathogens such as E. coli have nonetheless developed specific mechanisms to bind and alter the intestinal mucosa.  This generates a dysfunction in nutrient absorption and promotes the massive fluid loss that accompanies the infection.  The causes of this alteration are the effectors, proteins injected by the bacterium into the human cell during infection to alter intestinal cell function.

The EPEC strains, those studied by the researchers, are a category of bacteria that mainly affects young children.  This strain distinguishes itself from others by producing a lesion in the intestinal tissues called A/E (attachment and effacement), which destroys the microvilli of the intestinal mucosa.

This E. coli strain injects a large group of proteins into the intestinal cell, to manipulate cellular functions to the benefit of the infection.  These proteins are the effectors.  All of the EPEC bacteria have a group of effectors, responsible for the main damage to the mucosa, which are found at a point in their genome termed LEE (locus of enterocyte effacement).

There is also a larger group of effectors, scattered in different parts of the bacterium's genome, whose number varies depending on the strain.  These are the non-LEE effectors.  What the research team led by the CSIC found is that for the A/E lesion to be produced in the intestinal tissue, action of the non-LEE effectors is necessary.

"These results allow us to better understand the process of infection and damage to the intestinal barrier and will allow us to develop vaccines based on attenuated EPEC strains able to colonize the intestinal tissue without causing the acute symptoms of the infection," concludes Luis Ángel Fernández.

.

NOTE! This site uses cookies and similar technologies.

If you continue browsing or do not change browser settings, we consider your acepptance for using. Learn more

I understand

COOKIES POLICY

A cookie is a text file that is stored on your computer or mobile device via a web server and only that server will be able to retrieve or read the contents of the cookie and allow the Web site remember browser preferences and navigate efficiently. Cookies make the interaction between the user and the website faster and easier.

General information

This Website uses cookies. Cookies are small text files generated by the web pages you visit, which contain the session data that can be useful later in the website. In this way this Web remembers information about your visit, which can facilitate your next visit and make the website more useful.

How do cookies?

Cookies can only store text, usually always anonymous and encrypted. No personal information is ever stored in a cookie, or can be associated with identified or identifiable person.

The data allow this website to keep your information between the pages, and also to discuss how to interact with the website. Cookies are safe because they can only store information that is put there by the browser, which is information the user entered in the browser or included in the page request. You can not run the code and can not be used to access your computer. If a website encrypts cookie data, only the website can read the information.

What types of cookies used?

The cookies used by this website can be distinguished by the following criteria:

1. Types of cookies as the entity that manages:

Depending on who the entity operating the computer or domain where cookies are sent and treat the data obtained, we can distinguish:

- Own cookies: are those that are sent to the user's terminal equipment from a computer or domain managed by the editor itself and from which provides the service requested by the user.

- Third party cookies: these are those that are sent to the user's terminal equipment from a machine or domain that is not managed by the publisher, but by another entity data is obtained through cookies.

In the event that the cookies are installed from a computer or domain managed by the editor itself but the information collected by these is managed by a third party can not be considered as party cookies.

2. Types of cookies as the length of time that remain active:

Depending on the length of time that remain active in the terminal equipment can be distinguished:

- Session cookies: cookies are a type designed to collect and store data while the user accesses a web page. Are usually used to store information that only worth preserving for the service requested by the user at any one time (eg a list of products purchased).

- Persistent cookies: cookies are a type of data which are stored in the terminal and can be accessed and treated for a period defined by the head of the cookie, and can range from a few minutes to several years.

3. Cookies types according to their purpose:

Depending on the purpose for which the data are processed through cookies, we can distinguish between:

- Technical cookies: these are those that allow the user to navigate through a web page or application platform and the use of different options or services it exist as, for example, control traffic and data communication, identify the session, access to restricted access parts, remember the elements of an order, make the buying process an order, make an application for registration or participation in an event, use security features while browsing store content for dissemination videos or sound or share content via social networks.

- Customization cookies: these are those that allow the user to access the service with some general characteristics based on a predefined set of criteria in the user terminal would eg language, the type of browser through which you access the service, the locale from which you access the service, etc.

- Analysis cookies: they are those that allow the responsible for them, monitoring and analyzing the behavior of users of the web sites that are linked. The information gathered through such cookies are used in measuring the activity of web sites, application or platform and for the profiling of user navigation of such sites, applications and platforms, in order to make improvements function data analysis how users use the service.

Management tool cookies

This Website uses Google Analytics.

Google Analytics is a free tool from Google that primarily allows website owners know how users interact with your website. Also, enable cookies in the domain of the site in which you are and uses a set of cookies called "__utma" and "__utmz" to collect information anonymously and reporting of website trends without identifying individual users..

For statistics of use of this website use cookies in order to know the level of recurrence of our visitors and more interesting content. This way we can concentrate our efforts on improving the most visited areas and make the user more easily find what they are looking for. On this site you can use the information from your visit for statistical evaluations and calculations anonymous data and to ensure the continuity of service or to make improvements to their websites. For more details, see the link below privacy policy [http://www.google.com/intl/en/policies/privacy/]

How to manage cookies on your computer: disabling and deleting cookies

All Internet browsers allow you to limit the behavior of a cookie or disable cookies within settings or browser settings. The steps for doing so are different for each browser, you can find instructions in the help menu of your browser.

If you decline the use of cookies, since it is possible thanks to the preferences menu of your browser or settings, reject, this website will continue to function properly without the use of the same.

Can you allow, block or delete cookies installed on your computer by setting your browser options installed on your computer:

- For more information about Internet Explorer click here.
- For more information on Chrome click here.
- For more information about Safari click here.
- For more information about Firefox click here.

Through your browser, you can also view the cookies that are on your computer, and delete them as you see fit. Cookies are text files, you can open and read the contents. The data within them is almost always encrypted with a numeric key corresponding to an Internet session so often has no meaning beyond the website who wrote it.

Informed consent

The use of this website on the other hand, implies that you paid your specific consent to the use of cookies, on the terms and conditions provided in this Cookies Policy, without prejudice to the measures of deactivation and removal of cookies that you can take, and mentioned in the previous section.