Structural and physical determinants of adenovirus assembly

CSanMartin

 

Carmen San Martín

 Group Leader

 Contact

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Research summary

We are interested in the structural and physical principles that govern assembly and stabilisation of complex viruses. As a model system we use adenovirus, a challenging specimen of interest both in basic virology and in nanobiomedicine. We approach the problem from an interdisciplinary point of view, combining biophysics, computational, structural and molecular biology techniques.
Accurate knowledge of adenovirus structure and biology is fundamental to both the discovery of anti-adenovirus drugs and the design of new, efficient adenoviral therapeutic tools.

 

Publications

 

Pérez-Berná AJ, Mangel WF, McGrath WJ, Graziano V, Flint J, San Martín C. Processing of the L1 52/55k protein by the adenovirus protease: a new substrate and new insights into virion maturation. J Virol 2014; 88:1513-1524

Pénzes JJ, Menéndez-Conejero R, Condezo GN, Ball I, Papp T, Doszpoly A, Paradela A, Pérez-Berná AJ, López-Sanz M, Nguyen TH, van Raaij MJ, Marschang RE, Harrach B, Benk, M, San Martín C. Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes and the first adenovirus with either one short or three long fibers per penton. J Virol 2014; 88:11304-11314

Pérez-Berná AJ, Marion S, Chichón FJ, Fernández JJ, Winkler DC, Carrascosa JL, Steven AC, Šiber A, San Martín C. Distribution of DNA-condensing protein complexes in the adenovirus core. Nucleic Acids Res 2015 43:4274-4283.

Condezo GN, Marabini R, Ayora S, Carazo JM, Alba R, Chillón M, San Martín C. Structures of Adenovirus Incomplete Particles Clarify Capsid Architecture and Show Maturation Changes of Packaging Protein L1 52/55k. Journal of Virology 2015 89:9653-9664.

Alvaro Ortega-Esteban, Gabriela N. Condezo, Ana J. Pérez-Berná, Miguel Chillón, Sarah J. Flint, David Reguera, Carmen San Martín, and Pedro J. de Pablo. Mechanics of Viral Chromatin Reveals the Pressurization of Human Adenovirus. ACS Nano. 2015 Oct 28

 

More Publications

 

PortadasSanMartin

 

fig1SanMartin

Adenoviruses (AdVs) are pathogens with particular clinical relevance in the immunocompromised population. They are widely used as vectors for gene therapy, vaccination and oncolysis. AdVs have one of the most complex icosahedral, non-enveloped capsids known. Their genome, a dsDNA molecule, is bound to large amounts of positively charged proteins that help condense it and form the core, which is confined within an icosahedral capsid composed of multiple copies of seven viral proteins. The final stage of adenovirus morphogenesis consists of proteolytic processing of several capsid and core proteins. The immature virus, which contains all precursor proteins, is not infectious due to an uncoating defect. Although more than 200 types of AdV have been isolated from a variety of vertebrates, most current knowledge of their biology is derived from a few human AdV.

fig2SanMartinRed

Our current research lines focus on the less understood aspects of AdV assembly, such as: how the viral genome is packaged into the capsid; how does maturation occur; what are the key elements modulating virion stability and mechanical properties; what is the organization of the non-icosahedral virion components. Finally, we explore the structure and assembly of AdVs belonging to scarcely characterized species and genera.


 

 

 

 

 GrupoSanMartin

 

Name
Position
Contact
Carmen San Martín Principal investigator
Gabriela N. Condezo Castro Postdoctoral scientist
Mercedes Hernando Pérez Postdoctoral scientist
Marta Pérez Illana Predoctoral scientist
Natalia Martín González Predoctoral scientist

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