structure
determination of adenovirus proteins
keywords: adenovirus,
fibre, gene therapy, vaccination, animal adenoviruses
Adenoviruses are used as experimental vaccine, gene therapy
and anti-cancer vectors. We recently solved and published the
structure of the receptor-binding domain of the porcine
adenovirus type 4 NADC-1 strain. This revealed a conventional
head domain, which is probably not involved in
receptor-binding, and a galectin domain containing two
carbohydrate binding domains in tandem. The
carbohydrate binding domains are organised face-to-face,
contrary to expectations. Sequence comparisons and published
biochemical data suggest that tandem galectin domains from
other species may also show this organisation. We also solved
the structure of the galectin domain in complex with several
different oligosaccharides. Above a picture of the binding
site, the complex with tri-N-acetyllactosamine is shown.
For two genera of adenoviruses, the Atadenoviridae and
Siadenoviridae, we solved the first examples, from Snake
Atadenovirus 1 and Turkey Siadenovirus 3, respectively. The
knowledge of different adenovirus fibre protein structures
will expand the "toolbox" of investigators looking to direct
adenovirus-based vectors to specific cells.
publications
- Menéndez-Conejero R, Nguyen TH, Singh AK, Condezo GN,
Marschang RE, van Raaij MJ, San Martín C (2017) Structure
of a reptilian adenovirus reveals a phage tailspike fold
stabilizing a vertebrate virus capsid. Structure 25,
1562-1573.
- Nguyen TH, Ballmann MZ, Do HT, Truong HN, Benkő M,
Harrach B, van Raaij MJ (2016) Crystal structure of raptor
adenovirus 1 fibre head and role of the beta-hairpin in
siadenovirus fibre head domains. Virol J 13, 106.
- Singh AK, Berbis MA, Ballmann MZ, Kilcoyne M, Menendez M,
Nguyen TH, Joshi L, Cañada FJ, Jimenez-Barbero J, Benko M,
Harrach B, van Raaij MJ (2015) Structure and Sialyllactose
Binding of the Carboxy-Terminal Head Domain of the Fibre from
a Siadenovirus, Turkey Adenovirus 3. PLoS One 10, e0139339.
- Nguyen TH, Vidovsky MZ, Ballmann MZ, Sanz-Gaitero M, Singh
AK, Harrach B, Benko M, van Raaij MJ (2015) Crystal structure
of the fibre head domain of bovine adenovirus 4, a ruminant
atadenovirus. Virol J 12, 81.
- Singh AK, Menéndez-Conejero R, San Martín C, van Raaij MJ
(2014) Crystal structure of the fibre head domain of the
atadenovirus snake adenovirus 1. PLoS One 9, e114373.
- Pénzes JJ, Menéndez-Conejero R, Condezo GN, Ball I, Papp T,
Doszpoly A, Paradela A, Pérez-Berná AJ, López-Sanz M, Nguyen
TH, van Raaij MJ, Marschang RE, Harrach B, Benkő M, San Martín
C (2014) Molecular characterization of a lizard adenovirus
reveals the first atadenovirus with two fiber genes and the
first adenovirus with either one short or three long fibers
per penton. J Virol 88, 11304-11314.
- Singh AK, Menendez-Conejero R, San Martin C, van Raaij MJ
(2013) Crystallization of the C-terminal domain of the fibre
from snake adenovirus 1, an atadenovirus. Acta
Cryst F69, 1174-1179.
- Singh
AK, Ballmann MZ, Benko M, Harrach B, van Raaij MJ (2013)
Crystallization of the C-terminal head domain of the
fibre protein from a siadenovirus, turkey adenovirus 3.
Acta Cryst F69, 1135-1139.
- Guardado-Calvo P, Muñoz EM, Llamas-Saiz AL, Fox GC, Kahn R,
Curiel DT, Glasgow JN, van Raaij MJ (2010) Crystallographic
structure of porcine adenovirus 4 fiber head and galectin
domains. J Virol 84,
10558-10568.
- Guardado-Calvo P, Llamas-Saiz AL, Fox GC, Glasgow JN, van
Raaij MJ (2009) Crystallization of the head and galectin-like
domains of porcine adenovirus isolate NADC-1 fibre. Acta Cryst F65,
1149-1152.
- Guardado-Calvo P, Llamas-Saiz AL, Fox GC, Langlois P, van
Raaij MJ (2007) Structure of the C-terminal head domain of the
fowl adenovirus type 1 long fiber. J Gen Virol 88, 2407-2416.
- Mitraki A, Papanikolopoulou K, van Raaij MJ (2006) Natural triple
beta-stranded fibrous folds. Adv. Protein Chem. 73 (Fibrous Proteins: Amyloids,
Prions and Beta Proteins), 97-124.
- Guardado Calvo P, Llamas-Saiz AL, Langlois P, van Raaij MJ
(2006) Crystallization of the C-terminal head domain of the
avian adenovirus CELO long fibre. Acta Cryst F62, 449-452.
- Mitraki A, van Raaij MJ (2005) Folding of beta-structured
fibrous proteins and self-assembling peptides. Meth. Mol
Biol. 300 (Protein Nanotechnology: Protocols, Instrumentation,
and Application), 125-140.
- Papanikolopoulou K, Teixeira S, Belrhali H, Forsyth
VT, Mitraki A, van Raaij MJ (2004) Adenovirus fibre shaft
sequences fold into the native triple beta-spiral fold when
N-terminally fused to the bacteriophage T4 fibritin foldon
trimerisation motif. J Mol
Biol 342, 219-227.
- van Raaij MJ, Mitraki A (2004) Beta-structured viral fibres:
assembly, structure and implications for materials design.
Curr Opin Solid State Mater Sci 8,
151-156.
- Mitraki A, Miller S, van Raaij MJ (2002) Review:
conformation and folding of novel beta-structural elements in
viral fiber proteins: the triple beta-spiral and triple
beta-helix. J Struct Biol 137, 236-247.
- Mitraki A, van Raaij M, Ruigrok R, Cusack S, Fernandez JF,
Luckey M (2001) Structure, folding and assembly of adenovirus
fibers. In: Self-assembling peptides systems in biology,
medicine and engineering. A Aggeli, N Boden, S Zhang, eds,
Kluwer Academic Publishers, Dordrecht, pp 221-234.
- Lortat-Jacob H, Chouin E, Cusack S, van Raaij MJ
(2001) Kinetic analysis of adenovirus fiber binding to its
receptor reveals an avidity mechanism for trimeric
receptor-ligand interactions. J Biol Chem 276, 9009-9015.
- Leissner P, Legrand V, Schlesinger Y, Ali Hadji D,
van Raaij M, Cusack S, Pavirani A, Mehtali M (2001) Influence
of adenovirus fiber mutations on viral encapsidation,
infectivity and in vivo tropism. Gene Ther 8, 49-57.
- van Raaij MJ, Chouin E, van der Zandt H, Bergelson
JM, Cusack S (2000) Dimeric structure of the coxsackievirus
and adenovirus receptor D1 domain at 1.7 A resolution. Struct
Fold Des 8, 1147-1155.
- van Raaij MJ, Mitraki A, Lavigne G, Cusack S(1999) A triple
beta-spiral in the adenovirus fibre shaft reveals a new
structural motif for a fibrous protein. Nature 401, 935-938.
- van Raaij MJ, Louis N, Chroboczek J, Cusack S (1999)
Structure of the human adenovirus serotype 2 fiber head domain
at 1.5 Angstrom resolution. Virology 232, 333-343.
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